We have identified and characterized the cis-acting sequences at the termini of the bacterial transposon Tn7 that are necessary for its transposition. Tn7 participates in two kinds of transposition event: high-frequency transposition to a specific target site (attTn7) and low-frequency transposition to apparently random target sites. Our analyses suggest that the same sequences at the Tn7 ends ...

Anterior-posterior axis patterning of the Drosophila embryo requires Nanos activity selectively in the posterior. This spatial asymmetry of Nanos is generated by the localization of nanos mRNA to the posterior pole of the embryo, where it is subsequently translated. Posterior localization of nanos is mediated by a complex cis-acting localization signal in its 3' untranslated region comprising s...

Expression of the asialoglycoprotein receptor (ASGR) by the human hepatocellular carcinoma cell lines HepG2 and HuH-7 in response to intracellular cGMP concentrations was previously shown to be regulated at the translational level (1). Stable transfection of COS-7 cells with deletion constructs encoding the asialoglycoprotein receptor H2b subunit localized the cGMPresponsive cis-acting element ...

Drug tolerance and withdrawal are insidious responses to drugs of abuse; the first increases drug consumption while the second punishes abstention. Drosophila generate functional tolerance to benzyl alcohol sedation by increasing neural expression of the slo BK-type Ca(2+) activated K(+) channel gene. After drug clearance this change produces a withdrawal phenotype-increased seizure susceptibil...

which are just module axioms (MM2) and (MM3). [In the case of the second axiom, we have to show that (r1r2)θ = (r1θ)r2. We are thinking of r′ as an element of the free R-module R, and r2 as an element of the ring R acting on this module.] The kernel of θ is {r ∈ R : mr = 0}. Clearly any element r ∈ Ann(M) satisfies this condition. Conversely, since m generates M, every element of M has the form...

The mobility properties of the Drosophila melanogaster P element in drosophilid and nondrosophilid species has been determined using a P-element mobility assay that is conducted transiently in insect embryos. P elements are mobilizable in all drosophilids tested, including species outside the genus Drosophila but not in the related Tephritidae (order: Diptera), although the P-element gene neces...

A binary classification problem is solved by acting on the combined evidence of several early vision modules. Each module gives an opinion as to the identity of an individual image element, and a consensus is reached by a trained Multi-Layer Perceptron (MLP).

We prove that the Dirichlet fundamental polyhedron for a cyclic group generated by a unipotent or hyperbolic element y acting on complex hyperbolic «-space centered at an arbitrary point w is bounded by the two hypersurfaces equidistant from the pairs w, yw and w,y~lw respectively. The proof relies on a convexity property of the distance to an isometric flow containing y.

The using possibility of the vortex light beam with topological charges 2 and 5 for the rotation of the micromechanical element (microturbine) was considered. The results of the full-scale experiment on the optical rotation of the special form microturbine were presented. We evaluated the force moment, acting the microturbine.

We previously reported that a 350-bp region of the adeno-associated virus (AAV) type 2 rep gene contains a cis-acting element responsible for the Rep-dependent replication of a transiently transfected rep-cap plasmid. In this study, we further report that replicated rep-cap sequences can be packaged into AAV capsids in the absence of the inverted terminal repeats.