Adenosine is a cellular metabolite with diverse derivatives that possesses wide range of physiological roles. We investigated the molecular mechanisms adenosine and cordycepin for their promoting effects in wound-healing process. The mitochondrial energy metabolism cell proliferation markers, cAMP responsive element binding protein 1 (CREB1) Ki67, were enhanced by cultured dermal fibroblasts. s...

Abstract Background FXa has recently been shown to active platelets via protease activated receptor. Direct inhibition of leads reduced platelet reactivity and arterial thrombosis. It is not known, if other coagulation factors besides FIIa directly activate platelets. In this study, we hypothesized that FXIa, FXIIa, Fα-XIIa, Fβ-XIIa FXIIIa Purpose inhibitors above mentioned are currently in cli...

Abstract Pro-opiomelanocortin (POMC) neurons in the hypothalamus play a role both control of metabolic state and sexual behavior. Along with fast neurotransmitters glutamate and/or GABA, POMC secrete cocaine- amphetamine-regulated transcript (CART) products gene, including β-endorphin α-melanocortin stimulating hormone (α-MSH). Published data from our lab demonstrate lack interest male mice whe...

Abstract Cutaneous mast cells underly numerous skin inflammatory processes. Our study reveals that the ablation of a subset sensory nonpeptidergic neurons expressing MrgprD increases expression cell gene module, including activating receptor, Mrgprb2, leading to increased reactivity MrgprB2 ligands, and cutaneous inflammation in several disease models, such as irritant dermatitis rosacea. Mrgpr...

There is increasing support for water molecules playing a role in signal propagation through G protein-coupled receptors (GPCRs). However, exploration of the hydration features GPCRs still its infancy. Here, we combined site-specific labeling with unnatural amino acids to molecular dynamics delineate how local ghrelin receptor growth hormone secretagogue (GHSR) rearranged upon activation. We fo...

Abstract Extracellular signaling molecules, among them the fibroblast growth factors (FGFs), enable cells to communicate with neighboring cells. Such molecules that receive and transmit a signal require specific tyrosine kinase receptors located at cell surface (fibroblast factor receptors, FGFRs). The binding of molecule its receptor results in dimerization conformational changes cytoplasmic p...

Introduction GPR30 is an intracellular transmembrane G protein-coupled receptor that mediates non-genomic estrogen signaling. The agonist G-1 modulates glucose homeostasis and vascular function. However, its impact on inflammation atherogenesis has not yet been investigated in the atherosclerotic apolipoprotein E-deficient(ApoE-/-) mouse model. Material methods ApoE-/- mice were fed a high-chol...

Introduction The activation of natural killer cells (NK) by their KIR (killer cell immunoglobulin-like receptor) is a potent defense system tumor cells. In this thesis we want to investigate the profile 16 inhibiting and activating KIRs in bloodserum patients with head neck squamous-cell carcinoma (HNSCC). Furthermore, calculate correlation between KIR-status overall survival (OS) HNSCC-patients.