Patients with degenerative disc disease (DDD) experience serious clinical symptoms, including chronic low back pain. A series of therapies have been developed to treat DDD, including physical therapy and surgical treatment. However, the therapeutic effect of such treatments has remained insufficient. Recently, stem cell‑based therapy, in which stem cells are injected into the nucleus pulposus i...

Steroids promote the myelination and regeneration in the peripheral nervous system. Whereas, little is known about the inducing effects by which the hormones exert their effects on Schwann cells differentiation. This could be revealed by the expression of Schwann cell markers in adipose-derived stem cells (ADSCs). The purpose of this study was to present the effects of progesterone and 17 β-est...

cell replacement therapy has provided the basis for future clinical applications to treat central nervous system injuries fallowing by car accidents. induced functional neurons are an option for replacing the lost neurons. the ability to monitor changes in membrane potential is a useful tool for studying neuronal function, but there are only limited options available at present. here, investiga...

Adult or somatic stem cells hold great promise for tissue regeneration. Currently, one major scientific interest is focused on the basic biology and clinical application of mesenchymal stem cells. Adipose tissue-derived stem cells share similar characteristics with bone marrow mesenchymal stem cells, but have some advantages including harvesting through a less invasive surgical procedure. Moreo...

Bone defect is one of the challenges for dentists in process healing bone tissue. can occur alveolar with etiology microorganisms and cyst expansion. In addition, cases defects are also often found treatment apex resection hemisection. Autologous graft a clinical gold standard defect. However, use has limited number growth factors produced. Tissue engineering latest method terms regeneration. t...

The potential use of stem cell based therapies for the repair and regeneration of various tissues offers a paradigm shift that may provide alternative therapeutic solutions for a number of diseases. The use of either embryonic stem cells (ESCs) or induced pluripotent stem cells in clinical situations is limited due to cell regulations and to technical and ethical considerations involved in gene...

Citation: Gojanovich AD, Gimenez MC, Masone D, Rodriguez TM, Dewey RA, Delgui LR, Bustos DM and Uhart M (2018) Human Adipose-Derived Mesenchymal Stem/Stromal Cells Handling Protocols. Lipid Droplets and Proteins Double-Staining. Front. Cell Dev. Biol. 6:33. doi: 10.3389/fcell.2018.00033 Human Adipose-Derived Mesenchymal Stem/Stromal Cells Handling Protocols. Lipid Droplets and Proteins Double-S...

objective(s): sertoli cells support in vivo germ cell production; but, its exact mechanism has not been well understood. the present study was designed to analyze the effect of sertoli cells in differentiation of mouse embryonic stem cells (mescs) to germ cells.   materials and methods: a fusion construct composed of a stra8 gene promoter and the coding region of enhanced green fluorescence pro...

Junctional adhesion molecules (JAMs) are expressed in diverse types of stem and progenitor cells, but their physiological significance has yet to be established. Here, we report that JAMs exhibit a novel mode interaction biological activity adipose-derived stromal/stem cells (ADSCs). Among the JAM family members, JAM-B JAM-C were concentrated along cell membranes mouse ADSCs. not was broadly di...