An approach to broaden the product range of the ethanologenic, gram-negative bacterium Zymomonas mobilis by means of genetic engineering is presented. Gene alaD for L-alanine dehydrogenase (EC 1.4.1.1.) from Bacillus sphaericus was cloned and introduced into Z. mobilis. Under the control of the strong promoter of the pyruvate decarboxylase (pdc) gene, the enzyme was expressed up to a specific a...

OBJECTIVE To evaluate lead exposure among lead-acid battery workers in Korea, to evaluate in more detail the erythrocyte pyrimidine 5'-nucleotidase (P5N) test for lead exposure, and to evaluate the abnormal accumulation of erythrocyte pyrimidine nucleotides in the battery workers. METHODS Activity of P5N and other biological variables were examined in 66 exposed workers in a lead-acid battery...

Ampicillin sodium salt (AMP) is commonly and effectively used to prevent bacterial infection in algal culture, but the response of strains AMP has not been investigated. In this study, Chlorella sorokiniana was selected evaluate influence on algae. enhanced contents chlorophyll two fatty acids, myristic acid (C22:1N9) tetracosanoic (C6:0), inhibited growth, carotenoid production, 16 acids C. so...

The European standardized method for measuring porphobilinogen synthase (PBGS, EC 4.2.1.24) activity in peripheral erythrocytes is widely used in epidemiological studies of low-level Pb absorption. We have modified it to include activation with dithiothreitol (DTT). In children with concentrations of Pb in whole blood ranging from 0.29 to 1.93 mumol/L, we found the following statistically signi...

The band gap for 2D PhC with different modes and different lattice structures using FDTD have been computed. Three PBGs between the first and the second bands, between the second and the third bands and between the fourth and fifth bands are concluded. Simulation results shows that for band structure computation is of 1D PhC using FDTD, it is found that some additional solutions appear, which a...