نتایج جستجو برای: alamar blue test
تعداد نتایج: 877687 فیلتر نتایج به سال:
OBJECTIVE Intra-articular injection of local anesthetic and/or corticosteroid is an adjunct treatment for arthritic and inflammatory orthopedic conditions. Despite potential benefits, there is growing concern that these medications may cause significant morbidity, including potential toxicity to intra-articular chondrocytes and synoviocytes. DESIGN Twenty dogs underwent intra-articular inject...
We evaluated alamarBlue as a metabolic indicator in a standardized assay for the measurement of serum bactericidal activity (SBA) to Haemophilus influenzae type b (Hib) using sera containing natural and vaccine-induced anticapsular (polyribosylribitol phosphate) antibodies. SBA assays with a colorimetric and a fluorometric end point in the presence of alamarBlue were developed and compared to a...
The rise of resistant pathogens and chronic infections tolerant to antibiotics presents an unmet need for novel antimicrobial compounds. Identifying broad-spectrum leads is challenging due to the effective penetration barrier of Gram-negative bacteria, formed by an outer membrane restricting amphipathic compounds, and multidrug resistance (MDR) pumps. In chronic infections, pathogens are shield...
Cytotoxicity tests of zinc sulfide (ZnS) and cadmium sulfide (CdS) quantum dots (QDs) synthesized via all-aqueous process with various surface conditions were carried out with human endothelial cells (EA hy926) using two independent viability assays, i.e., by cell counting following Trypan blue staining and by measuring Alamar Blue (AB) fluorescence. The ZnS QDs with all four distinct types of ...
Raman microscopy is employed to spectroscopically image biological cells previously exposed to fluorescently labelled polystyrene nanoparticles and, in combination with K-means clustering and principal component analysis (PCA), is demonstrated to be capable of localising the nanoparticles and identifying the subcellular environment based on the molecular spectroscopic signatures. The neutral na...
Melanoma differentiation associated gene-7 (mda-7): a novel anti-tumor gene for cancer gene therapy.
BACKGROUND The mda-7 gene (melanoma differentiation associated gene-7) is a novel tumor suppressor gene. The anti-proliferative activity of MDA-7 has been previously reported. In this report, we analyze the anti-tumor efficacy of Ad-mda7 in a broad spectrum of cancer lines. MATERIALS AND METHODS Ad-mda7-transduced cancer or normal cell lines were assayed for cell proliferation (tritiated thym...
Bio-electrospraying is fast becoming an attractive tool for in situ cell delivery into scaffolds for tissue engineering applications, with several cell types been successfully electrosprayed. Bone marrow derived mesenchymal progenitor/stem cells (BMSC), which are an important cell source for tissue engineering, have not been explored in detail and the effect of electrospraying on their "stemnes...
Célkitűzés: Munkánk célja biopolimer bázisú 3D nyomtatható hidrogél előállításának, pásztázó elektronmikroszkópos (PEM)vizsgálatainak és biokompatibilitásának bemutatása.Anyagok módszerek: Ansys SpaceClaim (Ansys Inc, USA) modellező szoftver segítségével 1 2 mm vastagságúmintákat terveztünk, majd biodegradábilis metakrilált poli-γ-glutaminsav (MPGA) alapon hidrogéleket állítottunkelő sztereolit...
Complement C5a is a potent inflammatory chemoattractant and might be beneficial therapeutic target for the induction of an effective anti-tumour response. agonist antagonist modulation have been demonstrated to either promote or inhibit tumour development in EMT6 murine mammary cancer cells both vitro vivo studies. For studies Alamar Blue cell viability assay was used determination, immunofluor...
With the fall of the Socialist block in 1989, the Cuban national economy collapsed, followed by a severe scarcity in gasoline, materials and especially food. Of Alamar, a New Town socialist housing project under construction in East Havana, only the areas dedicated to dwelling were finished, leaving the neighbourhood without a defined public space or economic centre. Due to the limited transpor...
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