Isoamyl alcohol oxidase (IAAOD) was purified to apparent homogeneity on SDS-PAGE from ultrafiltration (UF) concentrated sake. IAAOD was a glycoprotein, a monomeric protein with an apparent molecular mass of 73 and 87 kDa, by SDS-PAGE and gel filtration on HPLC, respectively. IAAOD showed high substrate specificity toward C5 branched-chain alkyl alcohol (isoamyl alcohol), and no activity toward ...

Alcoholic liver disease (ALD) is an important worldwide public health issue with no satisfying treatment available since now. Here we explore the effects of recombinant human cytoglobin (rhCygb) on chronic alcohol-induced liver injury and the underlying mechanisms. In vivo studies showed that rhCygb was able to ameliorate alcohol-induced liver injury, significantly reversed increased serum inde...

The aim of this work was to investigate the antioxidant potential of some commonly used drugs (bromocriptine, haloperidol and azithromycin) on alcohol-induced ulcers in the rat. The following parameters were determined: content of reduced glutathione, activities of catalase, xanthine oxidase, glutathione reductase, glutathione peroxidase, peroxidase, and lipid peroxidation intensity. A battery ...

Due to increasing interest in natural vanillin, two enzymatic routes for the synthesis of vanillin were developed. The flavoprotein vanillyl alcohol oxidase (VAO) acts on a wide range of phenolic compounds and converts both creosol and vanillylamine to vanillin with high yield. The VAO-mediated conversion of creosol proceeds via a two-step process in which the initially formed vanillyl alcohol ...

Superoxide dismutase, ascorbic acid, glutathione and uric acid levels were decreased and xanthine oxidase, glutathione-s-transferase activities were increased in alcohol treated (2 g/kg body weight, once daily for 30 days) group. However, treatment with ethanolic extract of ginger (100 mg/kg, 200 mg/kg body weight, po, once daily for 30 days) these parameters came to normalcy showing the antiox...

Amination of non-activated aliphatic fatty alcohols to the corresponding primary amines was achieved through a five-enzyme cascade reaction by coupling a long-chain alcohol oxidase from Aspergillus fumigatus (LCAO_Af) with a ω-transaminase from Chromobacterium violaceum (ω-TA_Cv). The alcohol was oxidized at the expense of molecular oxygen to yield the corresponding aldehyde, which was subseque...

The structural specificity of amine oxidation by lysyl oxidase was investigated using kinetic and NMR spectroscopic analyses. Substrate efficiency increased with increasing molecular distance from the alpha-carbon of the aromatic moiety substituted on the aliphatic chains of a series of primary amines. The p-hydroxyl substituent of p-hydroxybenzylamine significantly increased kcat over that of ...

this research with the aims of microbial flora determination in pasteurized milk and it’s shelf life study based on microbial and physicochemical parameters were performed within 10 days. in this study, 60 pasteurized milk samples randomly were selected and then total count of bacteria and total psychrophilic bacteria count were performed. to investigate microbial flora of pasteurized milk, col...

Unique dependence on the nature of metal salt and reaction conditions for coordination assembly reactions of varying architecture and nuclearity have been identified in V-shaped [Co3L4] and planar disc-like [Co7L6] compounds: [CoL2(μ-L)2(μ-OH2)2(CF3CO2)2] (1) and [Co(μ-L)6(μ-OMe)6]Cl2 (2) (HL = 2-{(3-ethoxypropylimino)methyl}-6-methoxyphenol). At room temperature varying reaction conditions, co...

Vanillyl-alcohol oxidase (VAO) is member of a newly recognized flavoprotein family of structurally related oxidoreductases. The enzyme contains a covalently linked FAD cofactor. To study the mechanism of flavinylation we have created a design point mutation (His-61 --> Thr). In the mutant enzyme the covalent His-C8alpha-flavin linkage is not formed, while the enzyme is still able to bind FAD an...