Mango (Mangifera indica cv. Ataulfo) peel contains bound phenolics that may be released by alkaline or acid hydrolysis and may be converted into less complex molecules. Free phenolics from mango cv. Ataulfo peel were obtained using a methanolic extraction, and their cellular antioxidant activity (CAA) and permeability were compared to those obtained for bound phenolics released by alkaline or a...

The biosynthesis and post-translational modification of placental alkaline phosphatase were studied in human choriocarcinoma cells, JEG-3. Pulse-chase experiments with [35S]methionine demonstrated that placental alkaline phosphatase was synthesized as a major precursor form with Mr 63,000, which was then converted to a mature form with Mr 66,000, by processing of its N-linked oligosaccharides f...

alkaline phosphatase closely resembled that extracted from microsomes of human liver in some physicochemical respects. The specific activities of these enzymes in the complex were found to be of similar order of magnitude as compared with those of the same enzymes in plasma membranes isolated from the human liver. From the precipitate formed by incubating the complex with its antibody, all the ...

Addition of alkaline phosphatase to rat kidney cytosol diminishes the ability of the mineralocorticoid receptor (MR) to bind aldosterone in a time-, temperature- and concentration-dependent form. A variety of phosphatase inhibitors, including levamisole, are effective in preventing this inactivation. On the other hand, when the steroid-receptor complex is incubated in the presence of alkaline p...

Herpes simplex virus type 1 (HSV-1) DNA replication intermediates exist in a complex nonlinear structure that does not migrate into a pulsed-field gel. Genetic evidence suggests that the product of the UL12 gene, termed alkaline nuclease, plays a role in processing replication intermediates (R. Martinez, R. T. Sarisky, P. C. Weber, and S. K. Weller, J. Virol. 70:2075-2085, 1996). In this study ...

In this study, partial least square (PLS), a multivariable analysis, was used to simultaneously quantitatively evaluate the effects of variables related three pretreatments (alkaline, hot water and acid) biomass properties poplar, salix corncob. The results showed that type most important variable influencing enzymolysis reducing sugar yield (ERSY). compositions affected ERSY more than pretreat...

We developed an HT-2 toxin-specific simple ELISA format with a positive read-out. The assay is based on an anti-immune complex (IC) scFv antibody fragment, which is genetically fused with alkaline phosphatase (AP). The anti-IC antibody specifically recognizes the IC between a primary anti-HT-2 toxin Fab fragment and an HT-2 toxin molecule. In the IC ELISA format, the sample is added together wi...

We present a simple method for sequential chemiluminescent detections of two different DNA loci on a single Southern blot. First, an enzyme-linked DNA probe for a unique sequence is detected with a horse-radish peroxidase (HRP) substrate followed by the detection of another enzyme-linked DNA probe for a different unique sequence with an alkaline phosphatase (AP) substrate that simultaneously in...

Detailed mineralogical analyses in areas with surface hydrothermal alteration zones associated recent volcanism (<1 Ma) the Central Andean Volcanic Zone could provide key information to unravel presence of hidden geothermal systems. In Cordón de Inacaliri Complex, a field an estimated potential ~1.08 MWe·km?2 has been recently discovered. this work, we focus on and discharge products area, a...