Matrix vesicles (MV) can be readily isolated from culture media of chicken growth plate hypertrophic chondrocytes grown in primary culture. The chondrocytes maintain normal morphology and synthesize type I1 collagen throughout the culture period. The culturederived MV are morphologically indistinguishable from MV seen in situ and are rich in alkaline phosphatase. Formation of alkaline phosphata...

The soil samples were collected and were primarily screened for isolation of amylase producing fungi. Among the isolated fungi, amylase producing isolates were identified by growing on starch agar media. The isolates exhibiting the maximum zone of clearance on starch agar media by iodine were identified and were subcultured on potato dextrose agar (PDA). The isolates were morphologically charac...

Fungal isolates were screened for alkaline protease production on casein containing agar plates and identified by clear zones of casein hydrolysis around colonies. Totally 141 fungal isolates were used in primary screening of alkaline protease and out of these, only 108 test fungi were found to produce alkaline protease activity as indicated by production of zone of casein hydrolysis around the...

Most protein-based affinity chromatography media are very sensitive towards alkaline treatment, which is a preferred method for regeneration and removal of contaminants from the purification devices in industrial applications. In a previous study, we concluded that a simple and straightforward strategy consisting of replacing asparagine residues could improve the stability towards alkaline cond...