The protein contents and binding modes of immunoglobulin-amylase complexes were investigated. Amylase staining after immunoelectrophoresis permitted identification of IgA-lambda type in three cases, IgA-kappa type in two cases and IgG-kappa type in one case. The precipitin line of Fab fragment of IgA in four patient's sera after papain digestion was found to have amylase activity. Sucrose densi...

In maltose syrup production, one of the critical processing stages is starch conversion process. During this process, reaction time and enzyme concentrations are two important parameters to obtain standard sugar spectrum. The purpose study is; i) find optimum during ii) determine process control dynamic in production. different amounts beta alpha-amylase enzymes (0.10, 0.15, 0.20 0.25 ml β-amyl...

The interaction between two phytohormones, gibberellins (GA) and abscisic acid (ABA), is an important factor regulating the developmental transition from seed dormancy to germination. In cereal aleurone tissue, GA induces and ABA suppresses the expression of alpha-amylases that are essential for the utilization of starch stored in the endosperm. In this work, the signaling pathways mediated by ...

When embryonated and de-embryonated cotyledons of cowpea (Vigna unguiculata L.) were kept for germination, only embryonated cotyledons (EC) developed into seedlings. alpha-Amylase activity appeared late in de-embryonated cotyledons (DEC), but increased and matched with that of EC on 4th day, and thereafter started declining. A higher content of reducing sugars may be one of the factors in down ...

Human serous-type ovarian tumors contain an acidic isoenzyme of amylase. Previous attempts at purification of tumor amylases have yielded preparations contaminated with other proteins. The purification scheme presented here incorporates an affinity-chromatography procedure, with use of cycloheptaamylose linked to epoxy-activated Sepharose, that is specific for alpha-amylase (EC 3.2.1.1). Purifi...

To study the effect of inserted peptides on the secretion and processing of exported proteins in Bacillus subtilis and Escherichia coli, pBR322-derived DNA fragments coding for small peptides were inserted between the DNA coding for the 31 amino acid B. subtilis alpha-amylase signal peptide and that coding for the mature part of the extracellular thermostable alpha-amylase of B. stearothermophi...

The role of heterotrimeric G proteins in gibberellin (GA) induction of a-amylase gene expression was examined in wild oat aleurone protoplasts. Mas7, a cationic amphiphilic tetradecapeptide that stimulates GDP/GTP exchange by heterotrimeric G proteins, specifically induced alpha-amylase gene expression and enzyme secretion in a very similar manner to GA1. In addition, Mas7 stimulated expression...