Hepatic cholesterol synthesis in rabbits, as measured by the incorporation of C(14)-labelled acetate, was inhibited by addition of non-toxic amounts of vanadyl sulfate (0.05 per cent VOSO(4).2H(2)O) to the diet. This diet reduced excess aortic cholesterol in cholesterol prefed rabbits when fed during the 6 weeks immediately following a 4 week period of cholesterol feeding.

A quick and direct method for measuring tocopherols (alpha, beta+gamma and delta) in vegetable oils has been developed using RP-HPLC with UV detection. Previous extraction of tocopherols is not required. The oil is diluted in hexane and an aliquot is mixed with ethanol containing an internal standard (alpha-tocopherol acetate). The chromatographic system consists of an ODS-2 column with a metha...

The present study was carried out to evaluate the influence of increasing amounts of dietary polyunsaturated fatty acids (PUFA) and alpha-tocopheryl acetate (alpha-TA) supplementation on lipid oxidation of raw and cooked thigh meat stored under refrigeration. One hundred ninety-two female, 1-d-old, broiler chickens were randomly distributed into 16 experimental treatments resuIting from the com...

The aims of this research was to investigate effect alpha-tocopherol on spermatozoa death in form apoptosis and necrosis rats (Rattus norvegicus) exposed 2,3,7,8- tetrachlorodibenzo-p-dioxin. Male were administered TCDD experimental groups. Five groups a combination alpha- tocopherol designed as follows; 0.5 ml corn oil (control negative group, K-), 700 ng/kg/d (treatment control), 77 (Group P1...

Various extracts of local marine red algae, Acanthophora spicifera were evaluated for antioxidant activity, total phenolic content and in-vitro toxicity. The antioxidant activity was measured using DPPH (1, 1-diphenyl-2-picrylhidrazyl) method, while Folin-Ciocalteu method was used for the total phenolic content. The cytotoxicity of the extracts were evaluated using invitro brine shrimp lethalit...

For efficient coupling of droplet-based microfluidics with mass spectrometry (MS), a spyhole drilled on the top of a microchip is used to sample the passing droplets by electrostatic-spray ionization (ESTASI) MS. The technique involves placing an electrode below the chip under the spyhole and applying high-voltage pulses. Electrospray occurs directly from the spyhole, and the droplet content is...

The data presented in this paper indicate operation of different mechanisms for acetate oxidation by A. aerogenes, depending on the carbon source used for growth. The mechanism for citrate-grown cells appears to involve a conventional citric acid cycle, whereas acetate-grown cells appear to incorporate acetate carbon more readily via a dicarboxylic acid cycle.

Cell-free systems that catalyze the condensation of acetylcoenzyme A to form long chain fatty acids have been prepared from chicken liver by Wakil et al. (l), from yeast by Lynen (2), and from rat liver and brain by Brady, Bradley, and Trams (3, 4). In 1957, Stumpf and Barber (5) described a mitochondrial system isolated from avocado mesocarp that incorporated (Y-acetate into long chain fatty a...

A procedure is described for selection of temperature-sensitive mutants affecting fatty-acid synthesis based upon radiation suicide of wild-type organisms by tritiated acetate selectively incorporated into fatty acids. At 37 degrees , two of the mutants extensively incorporate fatty-acid supplements provided in the medium, and grow for extended periods only when a trans-unsaturated or a combina...

5-Ethynyl-1,3-benzenedicarboxylic acid (H2ebdc) reacted with lead(II) acetate trihydrate yields a 1D ladder network, [Pb(ebdc)(MeOH)]2·H2O (1). Removing crystals of 1 from the mother liquor results in a facile single crystal to single crystal transition, yielding 2D [Pb(ebdc)] net (2) with a change in space group from I2/a to P1̄.