The post-translational processing of pig small-intestinal aminopeptidase N (EC 3.4.11.2) was studied in organ-cultured mucosal explants. Exposure of the explants to swainsonine, an inhibitor of Golgi mannosidase II, resulted in the formation of a M,-160000 polypeptide, still sensitive to endo-ffi-N-acetylglucosaminidase H. Swainsonine caused only a moderate inhibition of transport of the enzyme...

Pseudomonas aeruginosa is an opportunistic bacterial pathogen that can cause fatal acute lung infections in critically ill individuals. Lung damage due to chronic infections in cystic fibrosis sufferers is the major cause of morbidity and mortality in this group. The bacterium produces various immunomodulatory products that enable it to survive in the lung. Innate and increasing resistance to a...

Robinson, Birnbaum, and Greenstein (1) in 1953 accomplished the isolation of renal aminopeptidase from fresh hog kidney by extracting the activity from the particulate fraction of kidney homogenate with I-butanol and thereafter fractionating between 50 and 75% saturation with ammonium sulfate at pH 7. About 30% of the final product was extractable by organic solvents, the extractable material a...

background: because of the lack of an effective and economical control strategy against malaria (the most devastating infectious disease in developing countries) transmission-blocking vaccines (tbvs) concept has been raised in recent years, promising a more efficient way to malaria control. tbvs aim at interfering and/or blocking pathogen development within the vector, halting transmission to n...

aminopeptidase [EC 3,4,11.1] from a plant source was rare.2'6) The cDNA sequence of a leucine aminopeptidase, which is an inducible component of the defense response in Io,copersicon esculentum, has been analyzed,'} and the enzymatic characteristics of this aminopeptidase have been studied.S} During screening for aminopeptidases from plant sources by using Leu:pNA as a substrate, we found a new...

In angiotensin type 1 receptor-blocked rats, renal interstitial (RI) administration of des-aspartyl(1)-angiotensin II (Ang III) but not angiotensin II induces natriuresis via activation of angiotensin type 2 receptors. In the present study, renal function was documented during systemic angiotensin type 1 receptor blockade with candesartan in Sprague-Dawley rats receiving unilateral RI infusion ...

Human cytomegalovirus (HCMV) infects cells by a series of processes including attachment, penetration via fusion of the envelope with the plasma membrane, and transport of the viral DNA to the nucleus. The details of the early events of HCMV infection are poorly understood. We have recently reported that CD13, human aminopeptidase N, a metalloprotease, is present on blood cells susceptible in v...