An extremely haloalkaliphilic archaeon Natrinema sp. SSBJUP-1 showing potent amylolytic and proteolytic activities was isolated from the saline alkaline Lonar Lake, India. Amylase and protease were active over the neutral to alkaline pH range from 6 to 9. Amylase was more stable over a narrower temperature range of 40-60C than the protease but had the higher optimum (55C) for activity. By contr...

The mashing step underpins the brewing process, during which endogenous amylolytic enzymes in malt, chiefly β-amylase, α-amylase, and limit dextrinase, act concurrently to rapidly hydrolyze malt starch fermentable sugars. With barley malts, is relatively straightforward, due part malted barley’s high enzyme activity, thermostabilities, gelatinization properties. However, beers also contain glut...

Abstract Pozol is a beverage made with maize dough that prepared after boiling the kernels in limewater, causing decrease soluble sugars, starch being main fermentable carbohydrate dough. Previously, Streptococcus infantarius ssp. 25124 ( Sii -25124) was identified as most amylolytic bacteria isolated this product. Analysis of -25124 enzymes revealed two amylases, cytoplasmic α-amylase 55.7 kDa...

This study investigates the mechanisms as well as strategies for purification and characterization of potential enzymes involved in pathogenesis of entomopathogenic fungi. The test strain of Verticillium lecanii that was screened, during the present investigation, proved to be an efficient producer of protein and polysaccharide degrading enzymes (amylase, protease, and lipase), hence indicating...

α-Amylase is one of the important enzymes in the starch-processing industry. However, starch processing requires high temperature, thus resulting in high cost. The high adsorptivity of α-amylase to the substrate allows this enzyme to digest the starch at a lower temperature. α-Amylase from Saccharomycopsis fibuligera R64 (Sfamy R64), a locally sourced enzyme from Indonesia, has a high amylolyti...

α-Amylases are widespread enzymes that catalyze endohydrolysis of long α-1,4-glucan chains such as starch and glycogen. The highest amylolytic activity was found in 5th instar nymphs and midgut of the predatory bug, Andrallus spinidens F. (Hemiptera: Pentatomidae). The α-amylase was purified following a three-step procedure. The purified α-amylase had a specific activity of 13.46 U/mg protein, ...

Starch degradation in barley endosperm provides carbon for early seedling growth, but the control of this process is poorly understood. We investigated whether endosperm cell wall degradation is an important determinant of the rate of starch degradation. We identified iminosugar inhibitors of enzymes that degrade the cell wall component arabinoxylan. The iminosugar 1,4-dideoxy-1, 4-imino-l-arab...

The great majority of seeds from higher plants fail to germinate under anoxic conditions. While the metabolic pathways operating during the aerobic process of seed germination are well described, there is only limited evidence concerning metabolic pathways operating under conditions of limited oxygen availability. This is despite the importance of carbohydrate availability for plant tolerance t...

In this study, a total of 1125 actinobacteria were isolated from the selected mangrove species: Avicennia marina, Rhizopora mucronata and Ceriops tagal three study stations viz., Minnie Bay, Carbyn’s Cove Burmanallah. Among these stations, highest number was recorded in (64.97%), followed by (25.51%) at Burmanallah minimum (9.51%) Bay. Maximum (40.44%) than other species marina (34.13%) (25.42%...