Cereal Chem. 65(5):424-427 The ratio of A chains to B chains of rice amylopectins from two pairs of action of pullulanase on the /3-limit dextrin and from A and B chains by the near isogenic lines differing in the waxy gene was determined by the action combined action of /3-amylase and pullulanase. Waxy and nonwaxy (lowof pullulanase and of /3-amylase plus pullulanase on the fl-limit dextrins a...

It has been previously demonstrated that linear amylose can be transformed with the Q enzyme from potatoes to a branched polysaccharide possessing the chemical and biochemical properties of amylopectin (l-3). A mechanism for its synthesis was first suggested by Bourne and Peat (4) in 1945. They postulated that glucose-l-phosphate is built up by phosphorylase to an unbranched chain, “pseudoamylo...

Pullulanase (EC 3.2.1.41) plays an important role in the specific hydrolysis of branch points in amylopectin. Enhancing its thermostability is required for its industrial application. In this study, rational protein design was used to improve the thermostability of PulB from Bacillus naganoensis (AB231790.1), which has strong enzymatic properties. Three positive single-site mutants (PulB-D328H,...

It has been well established that a certain amount of ingested starch can escape digestion in the human small intestine and consequently enters the large intestine, where it may serve as a carbon source for bacterial fermentation. Thirty-eight types of human colonic bacteria were screened for their capacity to utilize soluble starch, gelatinized amylopectin maize starch, and high-amylose maize ...

UNLABELLED Carbohydrate storage is an important element of metabolism in cyanobacteria and in the chloroplasts of plants. Understanding how to manipulate the metabolism and storage of carbohydrate is also an important factor toward harnessing cyanobacteria for energy production. While most cyanobacteria produce glycogen, some have been found to accumulate polysaccharides in the form of water-in...

The source strength and sink demand as well their interaction have been demonstrated to co-regulate the synthesis of starch determine grain quality, but knowledge underlying physiological mechanisms is limiting. An indica variety, Yangdao 6, a japonica Jinxiangyu 1, were planted with three treatments, including normal growth plant (CK), leaf-cutting (LC) spikelet-thinning (ST). transcript level...