purpose: to evaluate the properties of cultured limbal stem cells for corneal surface reconstruction. methods: specimens of limbal explants were prepared from the eye bank of i.r. iran. the explants were cultured and expanded on acellular amniotic membrane for 21 days. the cultured cells were evaluated for expression of connexin 43 and keratinine k3 by immunocytochemistry and expression of k12 ...

By immunocytochemistry and in situ hybridization at the electron microscopy level, and by the PCR technique, we have shown that HIV-1 binds and enters normal sperm; that viral particles, their antigens, and nucleic acid are present in sperm from HIV-1 infected men; and that such sperm can transfer HIV-1 like particles to normal human oocytes. We also present evidence that a galactosylceramide-l...

METHODS. ARPE-19 cells were used as a model to determine the effects of GS on the expression of the ICAM-1 gene upregulated by TNFor IFN, by Western blot analysis and semiquantitative reverse transcription polymerase chain reaction (RT-PCR). The activation and nuclear translocation of the nuclear factors NFB and STAT1 were evaluated by immunocytochemistry, Western blot analysis, and electrophor...

Hair regression and balding are distressing concerns for an increasing number of people due to changes in lifestyle and serious nutritional imbalances. Therapies for treatment of hair loss are needed. Among potential therapeutics, adenosine has been suggested as a potent regulator of hair growth. In this study, we investigated the effects of adeno...

Optimized Technique for for isolation of Endothelial cells Abstract The aim of the present study is to isolate endothelial cells from goat umbilical artery and to characterise them according to gross morphological and immunological criteria. A relatively simple, effective and less time consuming method was used for the first time to isolate and characterise goat umbilical artery endothelial cel...

AIM Tumor angiogenesis is a focus of molecularly-targeted therapies. This study investigated the effect of sodium butyrate (SB), a histone deacetylase inhibitor, on the synthesis of antiangiogenic and lymphangiogenic factors in oral squamous cell carcinoma. DESIGN Gene alterations in HSC-3 cells were assessed using cDNA microarrays before and after treatment with SB. The mRNA and protein expr...

METHODS. trpv1 mRNA expression in RGCs was probed with the use of PCR and TRPV1 protein localization through immunocytochemistry. Subunit-specific antagonism (iodo-resiniferatoxin) and agonism (capsaicin) were used to probe how TRPV1 activation affects the survival of isolated RGCs at ambient and elevated hydrostatic pressure ( 70 mm Hg). Finally, for RGCs under pressure, the authors tested whe...