17 The Flavivirus genus encompasses several arboviruses of public health significance 18 such as dengue, yellow fever, and Zika viruses. It also includes insect-specific flaviviruses 19 (ISFs) that are only capable of infecting insect hosts. The vast majority of mosquito-infecting 20 flaviviruses have been associated with mosquito species of the Aedes and Culex genera in the 21 Culicinae subfam...

Background: Insect symbionts have the potential to block the transmission of vector-borne diseases by their hosts. The advancement of a symbiont-based transmission blocking strategy for malaria requires the identification and study of Anopheles symbionts. Methods: High throughput 16S amplicon sequencing was used to profile the bacteria associated with Anopheles gambiae sensu lato and identify p...

Phylogenetic relationships among species of the Myzorhynchella Section of Anopheles (Nyssorhynchus) were investigated using the nuclear ribosomal DNA second internal transcribed spacer (ITS2), the nuclear whitegene and mitochondrial cytochrome oxidase subunit I (COI) regions. The recently described Anopheles pristinus and resurrected Anopheles guarani were also included in the study. Bayesian p...

Mosquitoes of the Anopheles gambiae complex display strong preference for human bloodmeals and are major malaria vectors in Africa. However, their interaction with viruses or role in arbovirus transmission during epidemics has been little examined, with the exception of O'nyong-nyong virus, closely related to Chikungunya virus. Deep-sequencing has revealed different RNA viruses in natural insec...

There is a lack of data on the Anopheles fauna, its biology and the roles played by different vector species in the transmission of malaria in the mount Cameroon region. The biting habits, feeding behaviour and entomological inoculation rates of different Anopheles species during the dry and rainy season were investigated. A total of 2165 Anopheles was collected, 805 in the rainy season and 136...

BACKGROUND All vectors of human malaria, a disease responsible for more than one million deaths per year, are female mosquitoes from the genus Anopheles. Evarcha culicivora is an East African jumping spider (Salticidae) that feeds indirectly on vertebrate blood by selecting blood-carrying female mosquitoes as preferred prey. METHODOLOGY/PRINCIPAL FINDINGS By testing with motionless lures made...

glutathione s-transferases (gsts) are a major family of detoxification enzymes which possess a wide range of substrate specificities. interest in insect gsts has primarily focused on their role in insecticide resistance. in this study, following world health organization (who) routine susceptibility test, dna was extracted from specimens of anopheles stephensi collected from the kazeroon distri...

Two new mosquito species belonging to the Hyrcanus Group, Anopheles (Anopheles) belenrae Rueda and An. (Ano.) kleini Rueda, are described from the Republic of South Korea, with illustrations of the larvae, pupae, adults, and male genitalia.

A total of 460 Anopheles funestus s.l. was collected inside human dwellings in Mwea, central Kenya. Of the 414 specimens that were positively identified, 1 was Anopheles leesoni and the others were Anopheles parensis. None of the 373 specimens tested were positive for Plasmodium falciparum or Plasmodium malariae sporozoites by enzyme-linked immunosorbent assay (ELISA). Of the 139 ELISA blood-me...

Knock down efficacy of commercially available insecticides against Anopheles gambiae Nicholas K. Makworo , Vincent O. Ochieng, Dorington Ogoyi, Richard W. Mukabana