Background: The unique expression of fibromodulin (FMOD) in patients with chronic lymphocytic leukemia (CLL) has been previously reported. Detecting FMOD in CLL patients using specific anti-FMOD mAbs might provide a promising method in detection, monitoring, and prognosis of CLL. Objectives: In this study, we aimed for producing specific antibodies agains...

Background: In spite of available, and sensitive screening assay for detection of hepatitis B virus surface antigen (HBsAg), occasional cases of post-transfusion hepatitis B virus infection are still observed. The aim of the present study was to assess the prevalence of positive anti hepatitis B core (anti-HBc) and presence of HBV-DNA in serum sample of healthy blood donors negative for both HB...

background: invariant natural killer cells (inkt) are an important immunoregulatory t cell subset. currently several flow cytometry-based approaches exist for the identifi-cation of inkt cells, which rely on using the 6b11 monoclonal antibody or a combina-tion of anti-vα24 and anti-vβ11 antibodies. objective: the aim of this study was to compare the ability of two flow cytometry-based methods f...

PURPOSE KRAS mutations are frequent driver mutations in multiple cancers. KRAS mutations also induce anti-EGFR antibody resistance in adenocarcinoma such as colon cancer. The aim of this study was to overcome anti-EGFR antibody resistance by coupling the antibody to KRAS-specific siRNA. EXPERIMENTAL DESIGN The anti-EGFR antibody was chemically coupled to siRNA. The resulting complex was teste...

Background Inflammatory muscle diseases are systemic inflammatory disorders that affect not only muscles but also skin, joints, lungs, and heart. Dermatomyositis (DM) polymyositis (PM) characterized by the production of antibodies. Measurement these antibodies is useful for diagnosis, classification disease type, evaluation activity, prognosis prediction, determination therapeutic strategy. How...

Platelets were obtained by differential centrifugation from Group A donors and were incubated with Na2-51Cr04. After washing to remove excess chromium, the platelets were exposed for one hour to Anti A antibody which showed agglutinating activity only. Chromium release was no greater than that of controls. Hemolytic Anti A in the presence of rabbit complement released 15 percent more chromium t...

background:  cytotoxic t lymphocyte–associated antigen 4 (ctla-4) molecules are expressed on t-cells and inhibit their function by inhibiting activation of subsequent t-cell molecular pathways. blocking of ctla-4 inhibits the growth of malignant tumor cells. anti-ctla-4 monoclonal antibodies activate the immune system against cancer. due to several advantages of single-chain antibodies (scfvs) ...