Doubt has sometimes been cast upon the protective effect of rabies antibodies in serum. Animals and humans suffering from fatal rabies often produce high antibody titres, while rabies cases are also observed in vaccinated animals. Cellular immunity is also largely involved in protection. Nevertheless, a large number of laboratory experiments and field observations clearly demonstrate that cats ...

Regulations governing international movements of domestic carnivores from rabies-infected to rabies-free countries have recently been loosened, with the adoption of a system that combines vaccination against rabies and serological surveillance (neutralising antibody titration test with a threshold of 0.5 UI/ml). Since 1993, the Research Laboratory for Rabies and Wild Animal Pathology in Nancy, ...

Dengue viruses (DENVs) are members of Flaviviridae family, which are associated with human disease. The envelope (E) protein plays an important role in viral infection. However, there is no effective antibody for clinical treatment due to antibody dependent enhancement of infection. In this study, using Systematic Evolution of Ligands by Exponential Enrichment (SELEX), we demonstrated the first...

Skom and Talmage have demonstrated a non-precipitating antibody against insulin in the sera of insulin-treated diabetics. But their method of determining the antibody is not quantitative. In the present study, a titration of the insulin binding activity of anti-insulin was performed more quantitatively using 1311-labeled insulin and antiguinea pig r-globulin rabbit sera under the condition of i...

Immunoglobulin G to human herpesvirus 6 (HHV-6) and cytomegalovirus (CMV) in sera from solid organ recipients was measured by an enzyme-linked immunoassay (ELISA) before and after transplant. The HHV-6 ELISA was developed from glycine extracts of HHV-6-infected and uninfected HSB-2 cells. At a serum dilution of 1:500, 80 (91%) of 88 recipients were seropositive for HHV-6 before transplant, whil...

After kaolin treatment of fetal rabbit serum, 7S antibody titers were reduced more than 19S titers. This reduction was less when the kaolin treatment was performed at pH 9.0 than when it was performed at pH 7.3. A modification of the kaolin treatment of sera for use in the hemagglutination-inhibiting antibody titration, in which the hemagglutination reaction is performed at a neutral pH, is rec...

Polysilicon nanowire biosensors have been fabricated using a top-down process and were used to determine the binding constant of two inflammatory biomarkers. A very low cost nanofabrication process was developed, based on simple and mature photolithography, thin film technology, and plasma etching, enabling an easy route to mass manufacture. Antibody-functionalized nanowire sensors were used to...

Exposure of both mammalian and reptilian cells in tissue culture to different strains of fixed rabies virus resulted in a carrier type of infection. No cytopathic effect was observed in either type of culture; infected cultures could be maintained by cell transfer for unlimited numbers of passages. A consistent pattern of cyclically rising and falling levels of viral infection was observed by f...

The Technicon autoanalyzer system used for the detection of homologous human hemagglutinins has been modified for the detection of hemagglutinins to sheep red blood cells. Antibody quantitation was obtained by plotting the optical density (OD) readings of peak heights of a serially diluted reference serum versus the serum dilutions. When the OD of the peak height of an unknown sample fell withi...

The identification of specific cell surface glycoprotein receptors for Arg-Gly-Asp-containing extracellular matrix proteins such as fibronectin has focused attention on the role of gangliosides in this process. Is their involvement dependent or independent of the protein receptors? In attachment assays with cells from a human melanoma cell line, titration experiments with an antibody (Mel 3) wi...