Mucosal-associated invariant T cells (MAIT cells) represent a potential therapeutic target as they can tune or enhance immune responses. They recognise and become activated by antigens, presented the monomorphic MHC-I related molecule, MR1. To assess significance of MAIT in human diseases, better understanding cell-MR1-antigen interaction is imperative. Easy access to MR1 ligands activators hel...

The properties and sequence of an oligomeric antigen of Treponema pallidum are presented. Antigen C1-5 assembles into oligomers of 140,000 and greater. The nucleotide sequence predicts an open reading frame for a protein monomer of 19,400, confirmed by amino-terminal sequencing of the recombinant antigen.

The objective of this study was to compare different antigen preparations to produce monoclonalantibodies against bovine leukaemia virus gp51SU. The four antigen preparations for immunization ofBALB/c mice were: CL: BLV-FLK cell lysate, UF: a fraction of CL (between 30 and 100 kDa), WVP: wholevirus particles and SP: with ion exchange chromatography, gp51SU was semipurified. A total of ninesucce...

background community acquired pneumonia (cap) is a common health concern and the main mortality factor worldwide. nowadays, legionella pneumophila is one of the most common microorganisms responsible for cap. we designed this study to investigate the antigen test as a useful, simple and rapid test for early diagnosis of legionella pneumophila. materials and methods we enrolled 118 patients (32 ...

The measurement of both immune complex-bound and free unbound tumor-associated antigen was evaluated independently on a panel of sera from colon cancer patients by radioimmunoassay (RIA). A monoclonal antibody (mAb 46.3) raised against secreted antigens from human colon cancer cells in vitro was utilized in the RIA. When circulating immune complexes alone were analyzed, the data demonstrated th...

Abstract We engineered a novel adenovirus with modified capsid for use as an integrated anticancer gene therapy and to circumvent pre-existing immunity. The adenovirus, SynBAd, when administered intravenously, induced antigen-specific CD8 T cell immunity in the liver of mice (C57BL/6) splenic population effector memory cells at levels equivalent those by human Adenovirus serotype 5 (Ad5). Altho...

The nephritogenic antigen, responsible for the immunogenic stimulus in experimental allergic glomerulonephritis induced with tubular antigen, has been identified as a renal tubular epithelial (RTE)-specific antigen and has been isolated in a relatively purified form. This antigen, RTE-alpha(5), is a distinct and antigenically specific lipoprotein of high density which is derived primarily from ...

Indirect immunofluorescence of certain human sera demonstrated an antigen(s) in the cytoplasm of 1--5% of the cells of a T-cell line, MT-1, from a patient with adult T-cell leukemia (ATL), which is endemic in southwestern Japan. The antigen was not detected in other human lymphoid cell lines, including six T-cell lines, seven B-cell lines, and four non-T non-B cell lines. The antigen did not sh...

An antigen that clearly marked T helper cells was first identified when the W 3 / 2 5 mouse monoclonal ant ibody was raised against rat thymocytes (1, 2). T h e W 3 / 2 5 antigen appears to be equivalent to human T 4 antigen, which is now called CD4 (3), and to mouse L 3 T 4 antigen (4-6). Hencefor th , we will call the rat ant igen "CD4 (W3/25) ." Antibodies to CD4 antigens inhibit T helper ce...

Abstract Immune responses to disease are complex and the only way understand such a response is consider behavior of multiple different cell types across both innate adaptive branches immune system. We developed Barcode Enabled Antigen Mapping (BEAM) technology enable easy enrichment antigen-specific cells. This fully integrated with 10x Genomics Chromium Single Cell Profiling Solution allow mu...