Chronic infection with the hepatitis B virus (HBV) can lead to liver failure and can cause liver cirrhosis and hepatocellular carcinoma (HCC). Reliable means for detecting and monitoring HBV infection are essential to identify patients in need of therapy and to prevent HBV transmission. Nanomaterials with defined electrical, optical, and mechanical properties have been developed to detect and q...

Introduction: Helicobacter pylori infection has various clinical features. One of the most common presentations of this infection is upper abdominal pain. Complications such as gastritis, gastric ulcer, gastric carcinoma and MALT lymphoma mandate early diagnosis of H.P infection by a low cost and non invasive manner. The aim of this study was to evaluate the diagnostic role of H.P stool antige...

This study examined circulating filarial antigen by monoclonal antibody Og4C3-enzyme-linked immunosorbent assay (ELISA) from 114 men with hydrocele, living in an endemic area. Nocturnal blood and hydrocele fluid were collected and examined for microfilaria. ELISA was performed on serum and hydrocele fluid for detection of antigen. Amongst 114 cases, 5(4.4%) showed microfilaria in blood but none...

the sip gene encoding for a conserved highly immunogenic surface protein of streptococcus agalactiae was amplified using polymerase chain reaction (pcr) and subcloned into prokaryotic expression vector pet32a (+) and expressed as a recombinant protein in e. coli bl21 (de3). an indirect enzyme linked immunosorbent assay (elisa) was developed using the purified sip protein as a coating antigen, w...

results the results showed that sensitivity and specificity were respectively 84.12% and 92% for‎ recombinant protein srs3 and 92% and 96% for rsag1. conclusions recombinant proteins srs3 and rsag1 are promising antigens in diagnostic assays for detection of specific antibodies against t. gondii. background infections by the protozoan parasite toxoplasma gondii (t. gondii) are widely prevalent ...

Malaria presents a diagnostic challenge in most tropical countries like India. Microscopy remains the gold standard for diagnosing malaria, but it is labour intensive and depends upon the skill of the examiner. Malaria rapid diagnostic tests (RDT’s) have been developed as an easy, convenient alternative to microscopy. The aim of this study was to correlate and compare the conventional diagnosti...

BACKGROUND Pneumococcal pneumonia can be diagnosed by the detection of capsular antigen in sputum, serum, pleural fluid, or urine using countercurrent immunoelectrophoresis and latex agglutination. In addition, quantitative cultures of bronchoalveolar lavage (BAL) fluid are also reliable for establishing the aetiology of pneumonia. This study investigated the value of rapid detection of pneumoc...

HIGHLIGHTS The main COVID-19 diagnostic methods in Brazil are updated. More than 80% of the approved products from other countries. tests based on detection nucleic-acid (15.8%), antigen (13%) and antibody (71.2%). most Brazilian federative units show an elevated percentage cases per samples tested.