Helichrysum sanguineum, Helichrysum pamphylicum, Helichrysum orientale, Helichrysum noeanum (Asteraceae) are medicinal plants. For centuries, they have been used as tea in Turkey because of their medicinal properties. So far no scientific evidence has been found in a literature survey regarding the genotoxic effects of these plants. This work evaluated the genotoxic effects on human lymphocyte ...

S, 'treptozotocin, an antibiotic produced from Streptomyces achromogenes, is now being used frequently for the production of experimental cataract." The exact mechanism of this cataract formation has not yet been determined. Streptozotocin has two distinct properties, either of which it was considered could be responsible for the cataract formation. First, Streptozotocin possesses the property ...

Ceratamine A and ceratamine B are heterocyclic alkaloids recently identified in a screen for compounds that arrest cells in mitosis. Treatment of breast carcinoma MCF-7 cells causes a concentration-dependent block of cell cycle progression exclusively at mitosis. In vitro studies with purified tubulin indicate that the ceratamines directly stimulate microtubule polymerization in the absence of ...

Through metabolic profiling of mutants and wild type of the endofungal bacterium Burkholderia rhizoxinica two novel rhizoxin derivatives with unusual nitrile substitutions were discovered. The nitrile groups result from a photochemical oxidative cleavage of the oxazolyl moiety. In vitro studies revealed that the photooxidation by singlet oxygen also takes place in the absence of a photosensitiz...

BACKGROUND Microtubule-targeting agents (MTAs) are a mainstay in breast cancer treatment, yet patient responses differ. The underlying mechanisms of these differences are unknown. While MTAs are mitotic inhibitors, recent evidence highlights that non-mitotic effects of these drugs can contribute to their anticancer effects. It is critical to identify the non-mitotic mechanisms that could contri...

Strains of Saccharomyces cerevisiae FL200 capable of growing on a solid medium containing a mixture of polyene macrolide antibiotics (nystatin, 40 micrograms/ml, amphotericin B, 40 micrograms/ml, pimaricin, 150 micrograms/ml and RP9971 antibiotic, 10 micrograms/ml) have been isolated after successive selection steps. The mutant strains, PR13 and PRC24, are 10 to 100 times more resistant than th...

Cell culture of neuronal tissues like spinal cord, auditory cortex, and frontal cortex all require special care. Neuronal tissue is dissected and later undergoes dissociation. After seeding onto plates or flasks, the trick is to keep them alive without exposure to too much stress and undoubtedly protecting them from contamination. Cultures must show signs of healthy neuronal cell growth and dev...

Paclitaxel is shown to be antiangiogenic at low doses, but the extent of these effects is not known. A mathematical model that describes tumor growth and response to treatment with a continuous, low dose treatment of the antimitotic drug Paclitaxel is considered. The model considers three populations: system cells, proliferating tumor cells, and tumor cells in a resting phase. A delay different...

By means of time-lapse microcinematography, metaphase arrest was studied in synchronized NHIK 3025 cells in contact with 5-chloropyrimidin-2-one (NY 3000) or vincristine. A dose-dependent prolongation of mitosis was found for both substances, and the fraction of cells able to escape mitotic arrest declined gradually as the concen tration of NY 3000 or vincristine was increased. Significant prol...