BACKGROUND This study aimed to investigate the effects of vitrification and slow freezing on actin, tubulin, and nuclei of in vivo preimplantation murine embryos at various developmental stages using a Confocal Laser Scanning Microscope (CLSM). MATERIAL/METHODS Fifty female mice, aged 4-6 weeks, were used in this study. Animals were superovulated, cohabitated overnight, and sacrificed. Fallop...

Ten, 4to 6-week-old BALB/c mice were randomly assigned to either control (non-vitrified, n = 5) or treatment (vitrified, n = 5) groups. Ovaries in the vitrified group were frozen sequentially by immersion into two vitrification solutions VS1: 10% ethylene glycol (EG) + 10% DMSO in holding medium (TCM-199 + 20% FBS) and VS2: 20% EG + 20% DMSO in holding medium. After thawing at 37 °C in 1.0 M su...

BACKGROUND Oocyte cryopreservation is one of the most important topics in the field of assisted reproductive technology to preserve women fertility, but relationship between cryopreservation and apoptosis is still a matter of debate. The present study was aimed to investigate the effects of vitrification on apoptosis in mouse oocytes by Cryotop method. METHOD A total of 200 germinal vesicle (...

The phase diagram of a system consisting of hard spheres with an attractive Yukawa interaction is computed by Monte Carlo simulations. Upon decreasing the range of attraction, we find the following scenarios: (a) a stable fluid-fluid and fluid-solid transition, a triple point with fluid-fluid-solid coexistence, and a metastable isostructural solid-solid transition (b) a fluid-fluid and isostruc...

Friable embryogenic tissue (FET) of oil palm was successfully cryopreserved by vitrification. After preconditioning the tissue on Murashige and Skoog (MS) medium supplemented with 0.25 M sucrose for 7 days, FET was dehydrated for 60 min at 0°C with a highly concentrated vitrification solution (PVS2) and plunged directly into liquid nitrogen gave the highest percentage of somatic embryo (SE) for...

Preimplantation genetic diagnosis and screening improves the chances of achieving a viable pregnancy, not only free of undesired single-gene defects but also aneuploidy. In addition, improvements in vitrification provide an efficient means of preserving embryos (blastocysts). By combining trophectoderm biopsy with recent improvements in vitrification methods, only those embryos that have proved...

The in vivo survival rate of rabbit morulae after vitrification in a mixture of dimethyl sulphoxide and ethylene glycol solution without protein supplement (WPS) was compared with two types of protein supplements: rabbit serum (RS) and bovine serum albumin (BSA). Significant differences were observed in the percentage of transferable embryos (undamaged embryos after devitrification, 80.4% versu...

Construction and Demolition Waste (CDW) originating from the rubble produced by 2016 seismic events in Marche Region (Central Italy) has been studied, focusing on its mineralogical chemical characteristics, to investigate recycling potentials as a component for eco-sustainable building material or glass industry. The aim was obtain full characterization of behaviour this at high T order determi...

OBJECTIVE This study was performed to compare the efficiency of slow freezing and vitrification based on survival, development to blastocysts, and cell numbers of blastocysts. Changes in embryonic gene expression in fresh and frozen-thawed embryos were also examined. METHODS Eight-cell stage embryos were collected from superovulated female BDF1 mice. The collected embryos were randomly divide...

The first pregnancy after vitrification of a human blastocyst (day 5 of embryo culture) was reported by Yokota et al. in 2000. Since then more attention has been given to the technique of vitrification and its safe application in ART. To the best of our knowledge, this is the first report of a clinical pregnancy resulting in a live birth from the transfer of a vitrified/ warmed human blastocyst...