The neutral sugars and hexosamines in the seromucoid fraction of normal human serum were identified and determined simultaneously by gas-liquid chromatography of their aldononitrile acetates. Besides the sialic acid, normal seromucoid contained fucose, arabinose, mannose, glucose, galactose, glucosamine and galactosamine. The presence of arabinose and glucose, not previously reported in seromuc...

The AraC protein, which regulates the L-arabinose operons in Escherichia coli, was dissected into two domains that function in chimeric proteins. One provides a dimerization capability and binds the ligand arabinose, and the other provides a site-specific DNA-binding capability and activates transcription. In vivo and in vitro experiments showed that a fusion protein consisting of the N-termina...

L–Arabinose-based coordination polymers were synthesized by the oxidation of L–arabinose using warm HNO3 to form arabinaric acid and allowing the resulting arabinaric acid to react with the different metal ions such as zinc(II), lead(II), copper(II), iron(III), and chromium(III). Only zinc(II) and lead(II) ions produced crystalline coordination polymers suitable for analysis while copper(II) pr...

L-Arabinose is a useful sugar in the food industry. We demonstrate here simple methods for refining arabinan polysaccharides by alcohol extraction from prune, Prunus domestica L., as a source of L-arabinose. Alcohol-soluble polysaccharides were purified from a solution of prune extracted by 80% ethanol. After fractionating the polysaccharides by ion-exchange chromatography, arabinans were ident...

Orally administered vaccine bacteria usually persist for a period of time in the intestinal tracts of immunized individuals, and are excreted in feces to the environment resulting in a potential biosafety issue. The releasing risk can be minimized by immediate elimination of the persistent vaccine bacteria once adequate protective immune responses have been elicited by the vaccine bacteria. In ...

An enzyme, D-phosphoarabinoisomerase, which stoichiometrically interconverts D-arabinose 5-phosphate and Dribulose S-phosphate, was isolated from Escherichia coli. At equilibrium, 75% of D-arabinose-5-P and 25% of D-ribulose-5-P are found. This unstable enzyme does not show any requirement for added cofactor, and is inhibited by Mn++, Co++, Zn+f, Cd++, p-chloromercuribenzoate, and phosphate. Th...

This study examined the effects of L-arabinose on mouse intestinal microbiota and urinary isoflavonoids. Male mice were randomly divided into two groups: those fed a 0.05% daidzein-2.5% L-arabinose diet (AR group) and those fed a 0.05% daidzein control diet (CO group) for 28 days. The amounts of daidzein detected in urine were significantly lower in the AR group than in the CO group. The ratio ...