Since the observations of Kidder et al. (8) that 8-azaguanine selectively inhibits the development of three types of mouse neoplasms, many investi gators have examined the carcinostatic power of this compound on a variety of cancer tissues in different animal species. It has been found that 8-azaguanine is active against many additional malignant neoplasms (5, 6, 9, 10, 14, 16) but produces lit...

This paper describes the development of an HPLC-UV-MS method for quantitative determination of andrographolide and dehydroandrographolide in Andrographis Herba and establishment of its chromatographic fingerprint. The method was validated for linearity, limit of detection and quantification, inter- and intra-day precisions, repeatability, stability and recovery. All the validation results of qu...

The first report of a methylcholanthrene-in duced transplantable melanotic tumor in a Syrian golden hamster was published by Shubik and asso ciates (14). Spontaneous hamster melanomas have been reported by Fortner and Allen (1) and Greene (5). Shubik found that no metastases occurred even when the tumors attained a size of 4 cm. in diameter. Fortner and Allen, and Greene reported diffuse metast...

The pharmacokinetics and metabolism of ifosfamide was investigated in a group of 16 pediatrie patients (5 girls) aged 1-17 years. Each received a dose of 3 g/m2/day for up to 3 days by continuous infusion. Plasma and urine were collected, and concentrations of ifosfamide and its principal metabolites were determined by a quantitative high-performance thin layer chromatography method. During 3 d...

Epimedii Herba is a traditional medicinal herb used in Korea and China and exerts estrogenic activity. In this study, we investigated the effect of peripubertal administration of Epimedii Herba on pubertal development in female rats using a modified protocol of the rodent 20-day pubertal female assay. Female Sprague-Dawley rats (21 days old after weaning, 10 rats per group) were divided into fi...

Previous work showed that mice that had been thymectomized at 3 days of age tended, in later life, to develop a hyperplastic autoimmune gastritis and were also hypersusceptible to sarcogenesis by low dosages of 3-methylcholanthrene. In the present work, it was found that the hyperplastic autoimmune gastritis could be transferred with great effi ciency with spleen cells obtained from 3-mo-old do...

OBJECTIVE To make an useful identification method for the molecule of DNA on 3 herbs of Artemisia genus and compare the differences of the genes of Korean and Chinese species of Artemisia. METHODS Sequence of 3 herbs (Artemisia sacrorum Ledeb., Artemisia iwayomogi Kitam. and Artemisia capillaris Thunb.) was determined by PCR sequence system. DNA was extracted from rDNA/ITS (internal transcrib...

To assess the ability of traditional Chinese medicine Cistanches Herba extract (CHE) to prevent bone loss in the ovariectomized (OVX) rat, Cistanches Herba extract (CHE) was administered intragastrically to the rats. Female rats were anesthetized with pentobarbital sodium (40 mg kg(-1), i.p.), and their ovaries were removed bilaterally. The rats in the sham-operated group were anesthetized, lap...

BACKGROUND Artemisia frigida Willd. is an important Mongolian traditional medicinal plant with pharmacological functions of stanch and detumescence. However, there is little sequence and genomic information available for Artemisia frigida, which makes phylogenetic identification, evolutionary studies, and genetic improvement of its value very difficult. We report the complete chloroplast genome...

Background Virus infection is the main triggering event for the development of acute rhinosinusitis and human influenza A virus ranks among the most frequent viral causes of infection. Influenza neuraminidase, a key enzyme in viral replication, spread, and pathogenesis, is the primary target in prevention and treatment of influenza infection. Sinupret, a herbal medicinal product composed of Gen...