OBJECTIVE To evaluate hyperlipidaemia in patients with acute lymphoblastic leukaemia (ALL) receiving L-asparaginase. METHODS A case-control study carried out between October 2007 and October 2010 with 77 patients undergoing chemotherapy at a teaching children's hospital in Babol, Iran. Patients were treated with anti-leukaemic agents according to the protocols for standard-risk and high-risk ...

Over the past several decades, L-asparaginase, an important component of therapy for acute lymphoblastic leukemia (ALL), has typically been administered intramuscularly rather than intravenously in North America because of concerns regarding anaphylaxis. We evaluated the feasibility of giving polyethylene glycosylated (PEG)-asparaginase, the polyethylene glycol conjugate of Escherichia coli L-a...

A method is proposed for measuring the cytocidal effect of L-asparaginase on leukemic blood cells as a possible means of screening patients for L-asparaginase therapy. On the average, L-asparaginase was more toxic to blood lymphocytes from patients with chronic lymphocytic leukemia than to lymphocytes from normal blood. A positive sensitivity test was defined as one in which 65% of lymphocytes ...

L-asparaginase is an enzyme used as a chemotherapeutic agent, mainly for treating acute lymphoblastic leukemia. In this study, the gene of L-asparaginase from Zymomonas mobilis was cloned in pET vectors, fused to a histidine tag, and had its codons optimized. The L-asparaginase was expressed extracellularly and intracellularly (cytoplasmically) in Escherichia coli in far larger quantities than ...

This article comprises detail information about Lasparaginase, encompassing topic such as various sources of Lasparaginase, mechanism and properties of L-asparaginase. Also describe the production, cultivation and purification of Lasparaginase along with information about the application of Lasparaginase. L-asparaginase catalyzes the conversion reaction to convert asparagine to aspartic acid an...

BACKGROUND Erwinia asparaginase is antigenically distinct from E.coli-derived asparaginase and may be used after E.coli-derived asparaginase hypersensitivity. In a single-arm, multicenter study, we evaluated nadir serum asparaginase activity (NSAA) and toxicity with intravenously administered asparaginase Erwinia chrysanthemi (IV-Erwinia) in children and adolescents with acute lymphoblastic leu...

PATIENT Male, 51 FINAL DIAGNOSIS: Encephalopaty toxic Symptoms: Confusion • disorientation • drowsiness • fever MEDICATION L-asparaginase Clinical Procedure: - Specialty: Oncology. OBJECTIVE Unknown ethiology. BACKGROUND Novel therapies have improved survival in malignancies of lymphoid origin. This improvement, however, has been at the cost of chemotherapy-related toxicities. L-asparagin...

Aggressive nature killer cell leukemia (ANKL) is a mature NK-T cell lymphoma with worse prognosis, but optimal treatment is unclear. Therefore, we analyzed the efficacy of L-asparaginase-based regimens for ANKL patients. Twenty-one patients who received dexamethasone, methotrexate, ifosfamide, L-asparaginase, and etoposide (SMILE) or etoposide, ifosfamide, dexamethasone, and L-asparaginase (VID...

Bacterial asparaginase (L-Asparaginase amidohydrolase, E.C. 3.5.1.1) is a selective and highly effective chemotherapeutic agent extensively used over the world in first-line treatment of acute lymphoblastic leukemia (ALL), Hodgkin disease, acute myelocytic leukemia, acute melanomonocytic leukemia, chronic lymphocytic leukemia, lymphosarcoma, reticlesarcoma and melanosarcoma (Verma et al., 2007)...

Bacterial L-asparaginases, which are widely used in the antitumor therapy, act only as homotetramers, because their active sites are located at the interface between the subunits of the enzyme. Since salt bridges substantially stabilize L-asparaginase tetramers, we have supposed that oligomerization of bacterial L-asparaginase is a high-avidity process. This assumption was proved by bioinformat...