A cellulolytic enzyme was isolated from a commercial cellulase preparation form Aspergillus niger. A yield of about 50mg of enzyme was obtained per 100g of commerial cellulase. The isolated enzyme was homogeneous in the ultracentrifuge at pH 4.0 and 8.0, and in sodium dodecyl sulphate/polyacrylamide-gel electrophoresis but showed one major and two minor bands in disc gel electrophoresis. No car...

A cadmium tolerant strain of Aspergillus niger and a lead tolerant strain of Rhizopus arrhizus were studied for distribution of accumulated toxic heavy metals in the mycelia in comparison to wild type strains. Accumulated cadmium in the cell wall fractions of cadmium tolerant Aspergillus niger was more than the wild type strain. Cytosolic fraction contained the next highest load of metal. Simil...

Affinity precipitation is a simple, single plate separation process in which the complex of a smart macroaffinity ligand with the target protein (from a crude broth) can be selectively precipitated by application of a suitable stimulus. Alginate is a copolymer of guluronic acid and mannuronic acid residues and precipitates with Ca(2+) ions. It was found to bind to pectinase present in a commerc...

BACKGROUND Infectious Bursal Disease Virus (IBDV) causes a highly immunosuppressive disease in chickens and is a pathogen of major economic importance to the poultry industry worldwide. The VP2 protein is the major host-protective immunogen of IBDV and has been considered as a potential subunit vaccine against the disease. VP2 coding sequence was cloned in an inducible fungal vector and the pro...

Pectinase is used commercially in the clarification and extraction of fruit juice from different fruits. Green apples and Red apples obtained from the fruits section of a supermarket, Idiroko road, Ota were surface sterilized and inoculated with Aspergillus niger. The stock culture was subcultured on Sabouraud Dextrose agar plates and 72-hr-old culture of Aspergillus niger served as the inoculu...

The aim of the study was investigate antifungal activity of the various leaves extracs of Achyranthes aspera Linn.The aqueous,ethanol and methanol leaves extracts of Achyranthes aspera Linn. (Family:Amaranthaceae) were evaluated for antifungal activity against clinically important fungal spp viz. Candida albicans (MTCC 227), C.tropicalis (MTCC 750), C.krusei (ATCC 6258), C.kefyr (ATCC 4235), C....

BACKGROUND AND PURPOSE Pistacia atlantica, which belongs to the Anacardiaceae family, grows in the Zagrossian region of Iran. The aim of this study was to evaluate the antifungal properties of Pistacia atlantica and olive leaf extracts against different fungal species. MATERIALS AND METHODS In this study, we assessed the activities of olive leaf extracts and Pistacia atlantica leaf and fruit ...

The occurrence of mycotoxigenic species in peanuts is a major concern, and has been investigated depth for many years. However, most studies focus on the aflatoxigenic fungi, such as Aspergillus section Flavi. present study aimed to clarify Nigri, group that harbors capable producing ochratoxin A (OTA), which scarcely peanuts. total 52 peanut samples, collected field from storage, were analyzed...

Dr. Beatriz L. Gomez and colleagues described a new inhibition immunoassay for the diagnosis of histoplasmosis (1). My laboratory developed and extensively evaluated an antibody sandwich immunoassay for this purpose. It is important to contrast the two methods, as their performance characteristics differ. The characteristics of the assay developed in my laboratory are high sensitivity in dissem...

Citric acid yields of 98.7% (sugar consumption basis) were reached in shaker flasks with mutant UV-ET-71-15 of Aspergillus niger in a resin-treated sucrose medium of the following composition (g/100 ml): sucrose, 14.0; NH(4)NO(3), 0.20; KH(2)PO(4), 0.10; MgSO(4).7H(2)O, 0.025; and (mg/liter): FeSO(4), 0.15 to 0.75; ZnSO(4), 0.10; and CuSO(4), 0.01. Yields of 75% were obtained in medium with res...