Five hundred sputum samples of clinically diagnosed or suspected tuberculous patients were subjected for the isolation of mycobacteria. Of these 239 (47.8 percent) were culture positive and 204 (40.8 percent) smear positive. Of the smear positive specimens 39 (7.8 percent) could not propagate on Lowenstein jensen medium. From total isolates, 235 strains were of Myco. tuberculosis and remaining ...

Mycobacterial infections are rare in developed countries. Isolated involvement of the liver and biliary tree by mycobacterial infection is extremely rare. We report a case of a 45-year-old Caucasian female presenting with obstructive jaundice with a common bile duct stricture and multiple hypodense liver lesions raising suspicion of a metastatic cholangiocarcinoma. Percutaneous core biopsies of...

Interleukin-12 (IL-12) is involved in cellular immune responses against intracellular pathogens by promoting the generation of T naive in T helper 1 (Th1) cells and by increasing interferon-gamma (IFN-gamma) production from T and natural killer (NK) cells. A defective induction of a Th1 response may lead to a higher risk of infections, and, in particular, infections due to typical and atypical ...

vancomycin and cefepime was started, which was followed by rapid clinical improvement. She was discharged home on oral amoxicillin–clavulanic acid. Interestingly, 10 days later, all cultures obtained from the exit site at different time points revealed acid-fast bacilli later identified as Mycobacterium fortuitum . The decision was made to use oral clarithromycin and ciprofloxacin for 2 months....

Background: Sarcoidosis is a granulomatous multisystem disease of unknown etiology. It has recently been tired to detect Mycobacteria genome in biopsy specimens of patients with sarcoidosis by Polymorphism chain reaction method. Objective: To detect and identify Mycobacteria species in cutaneous lesions of the patients with sarcoidosis by PCR-RFLP. Patients and methods: 20 patients with clinica...

Two strains of fastidious mycobacteria were isolated from two patients with AIDS and clinical disease suggestive of Mycobacterium avium complex infection. Acid-fast bacilli were isolated from blood and bone marrow of both patients in BACTEC 12B and/or 13A media. The acid-fast bacilli failed to grow on subculture to routine Löwenstein-Jensen medium containing pyruvate and egg yolk agar. After se...

objective(s) in addition to several molecular methods and in particular 16s rdna analysis, the application of a more discriminatory genetic marker, i.e., 16s-23s internal transcribed spacer gene sequence has had a great impact on identification and classification of mycobacteria. in the current study we aimed to apply this sequencing power to conclusive identification of some iranian clinical s...

The identification of Mycobacteria in the species level has great medical importance. Biochemical tests are laborious and time-consuming, so new techniques could be used to identify the species. This research aimed to the comparison of biochemical and sequencing 16S rDNA gene methods to identify nontuberculous Mycobacteria in patients suspected to tuberculosis in Golestan province which is the ...

As immunologists, we are frequently asked to evaluate patients with recurrent infections. These infections can provide us with clues regarding what pathways might be aberrant in a given patient, e.g., specific pyogenic bacteria with Toll-like receptor problems, atypical mycobacteria with interferon gamma receptor autoantibodies, and Candida/staphylococcal infections with cellular immune abnorma...

This type of molluscoid lesion obliges us to rule out cryptococcosis, histoplasmosis, atypical mycobacteria, molluscum contagiosum, disseminated leishmaniasis, and leprosy.1,2,5,7 We draw attention to the fact that the patient was a young woman of childbearing age who came from an endemic area with favorable environmental conditions for the fungus. She did not present immunosuppression except f...