Microalgae have several applications in nutraceuticals, pharmaceuticals, cosmetics, biofuel production, and bioremediation, among other fields. Isolation purification are extremely important for obtaining axenic cultures of microalgae from different environments crucial their biotechnological applications, but it is not an easy task. In view the above, fundamental to know classical advanced tec...

In this protocol, we present the required materials, and the procedure for making modified C. elegans Habituation and Reproduction media (mCeHR). Additionally, the steps for exposing and acclimatizing C. elegans grown on E. coli to axenic liquid media are described. Finally, downstream experiments that utilize axenic C. elegans illustrate the benefits of this procedure. The ability to analyze a...

The freshwater cyanobacteria, Microcystis sp., commonly form large colonies with bacteria embedded in their mucilage. Positive and negative interactions between Microcystis species and their associated bacteria have been reported. However, the potential role of bacteria in the production and degradation of cyanobacterial secondary metabolites has not been investigated. In this study, a Microcys...

The behaviour, during the multicellular phase of the life-cycle, of amoebae of Dictyostelium discoideum grown indifferent media is described. Amoebal populations were marked by growthtemperature-sensitive genetic lesions which do not interfere with developmental phenomena. The fate of cell populations was determined by measuring the relative number of mutant and wild-type cells at various stage...

In this study we examined whether the maintenance of Trypanosoma cruzi by long-time in axenic culture produces changes in gene expression and antigenic profiles. The studies were made with a Dm30L-clone from a low-virulent strain and a non-cloned virulent EP-strain of T. cruzi. Both parasites were maintained, for at least seven years, by successive alternate passage triatomine/mouse (triatomine...