We describe the construction and immunobiological properties of a novel whooping cough vaccine candidate, in which the aroQ gene, encoding 3-dehydroquinase, was deleted by insertional inactivation using the kanamycin resistance gene cassette and allelic exchange using a Bordetella suicide vector. The aroQ B. pertussis mutant required supplementation of media to grow but failed to grow on an uns...

BACKGROUND Pertussis is believed to be widely underreported and under-recognized, particularly among adults. The aim of this study was to estimate the incidence of private practitioner-attended cough illness that could be attributed to Bordetella pertussis in adults aged ≥50 years in the US. METHODS Multiple linear regressions were employed to estimate the overall incidence of pertussis. Data...

OBJECTIVE To assess the impact of outbreaks of Bordetella pertussis infection on a tertiary care medical system. DESIGN Retrospective study. SETTING Academic tertiary care medical center and affiliated ambulatory care settings. SUBJECTS All patients and healthcare workers (HCWs) who were in close contact with patients with laboratory-confirmed cases of B. pertussis infection from October ...

In the Netherlands, as in many other western countries, pertussis vaccines have been used extensively for more than 40 years. Therefore, it is conceivable that vaccine-induced immunity has affected the evolution of Bordetella pertussis. Consistent with this notion, pertussis has reemerged in the Netherlands, despite high vaccination coverage. Further, a notable change in the population structur...

To investigate the fundamental nature of protective immunity to Bordetella pertussis , we studied intranasal immunization of adult mice with formalin-fixed B. pertussis (FFBP), followed by aerosol B . pertussis challenge. Mice given two doses of FFBP intranasally completely cleared a subsequent pertussis aerosol challenge from tracheae and lungs (defined as protection), but there was no correla...

BACKGROUND Pertussis is a vaccine modified disease in most age groups and hence subtle in its presentation. Current diagnostic approaches require relatively invasive sampling. AIM To determine the incidence of B. pertussis infection among people aged 5-49 years identified in primary care with acute persistent cough using an oral fluid based diagnostic test. DESIGN AND SETTING Active surveil...

Whooping cough is traditionally ascribed to Bordetella pertussis; however, Bordetella parapertussis can cause a similar clinical syndrome. This study describes an outbreak of B. parapertussis in Southeastern Minnesota and the United States (US) in 2014. This was a retrospective analysis of Mayo Clinic and Mayo Medical Laboratories patients who tested positive for B. parapertussis from 2012 to 2...

Clinical studies of component (“acellular”) pertussis vaccines have been undertaken in recent years, and several acellular vaccines have been used in Japan for 10 years. The Committee has reviewed these trials and related data and herein provides its assessment regarding the current status of the acellular vaccines and their possible use in the United States. The pertussis vaccines in current u...

Abstract Background Although Respiratory syncytial virus (RSV) is one of the common pathogens in children with pertussis and viral coinfection, clinical impact RSV infection on remains unclear. We compared characteristics sought differences between infants single Bordetella ( B. ) those coinfection. Methods enrolled 80 patients who were hospitalized Shenzhen Children’s Hospital from January 201...

To investigate the fundamental nature of protective immunity to Bordetella pertussis, we studied intranasal immunization of adult mice with formalin-fixed B. pertussis (FFBP), followed by aerosol B. pertussis challenge. Mice given two doses of FFBP intranasally completely cleared a subsequent pertussis aerosol challenge from tracheae and lungs (defined as protection), but there was no correlati...