Inorganic polyphosphate (polyP) is obtained by the polymerization of the terminal phosphate of ATP through the action of the enzyme polyphosphate kinase (PPK). Despite the presence of polyP in every living cell, a gene homologous to that of known PPKs is missing from the currently sequenced genomes of Eukarya, Archaea, and several bacteria. To further study the metabolism of polyP in Archaea, w...

one of the most important aspects in recombinant biologic production, based on gmp rules, is the accuracy of final product quality control, especially assessment of host cell macromolecules contamination rate in final product. the purification requirement can be eliminated when the yeast cell containing the recombinant protein is used as a host cell. it is possibile that the final product conta...

The effects of dietary supplementation of garlic (Allium sativum) extract on the growth and disease resistance of African catfish, Clarias gariepinus was evaluated. Also in-vitro evaluation of susceptibility of fish-borne multidrug resistant (MDR) pathogenic bacteria to aqueous extract of garlic was done. Aqueous garlic extract exhibited inhibitory activity against MDR bacteria and the degree o...

OBJECTIVE(S) Streptokinase (SKa) is an antigenic protein which is secreted by Streptococcus pyogenes. Streptokinase induces inflammation by complement activation, which may play a role in post infectious diseases. In the present study, recombinant streptokinase from S. pyogenes was produced and showed that recombinant SKa protein was recognized by infected human sera using Western blot analysis...

2,6-difluorobenzamides have been deeply investigated as antibacterial drugs in the last few decades. Several 3-substituted-2,6-difluorobenzamides proved their ability to interfere with bacterial cell division cycle by inhibiting protein FtsZ, key player of whole process. Recently, we developed a novel family 1,4-tetrahydronaphthodioxane benzamides, having an ethoxy linker, which reached sub-mic...

Poultry meat and eggs are important food products and the poultry-related industry is an economically important component of the agro-industry in the EU. The industry however is under pressure and faces many challenges. Most importantly, there is a demand for eggs and meat of high nutritional value and free of microbiological and chemical hazards. Unfortunately poultry eggs and meat are one of ...

objectives the overall goal of this study was to carry out a set of comparative analyses of arsr gene in plasmid r773 and bacterial chromosome from escherichia coli bl-21(de3). methods pdb and ncbi databases and chimera, mega4, clc main workbench software and 3d-jigsaw and embl-ebi servers were applied to perform this study. by using these software and servers, multiple analyses including deter...

a total of twenty seven bacterial strains were isolated from cankerous tissues of apricot, nectarine, peach, plum, sour cherry and sweet cherry trees in tehran province and identified as pseudomonas syringae pv. syringae (pss), the causal agent of the bacterial canker disease, on the basis of lopat (levan production, oxidase test, potato rot, arginine dihydrolase and tobacco hypersensitive ...

Paulownia tomentosa (Thunb.) Steud. is a very important hard woody plant, an extremely fast-growing tree and produce timber. Therefore, there is a demand to produce transgenic Paulownia plant resistant to bacterial infection. Microbial infection (especially bacterial one) is serious sever and cause a loss in plant productivity as they bear upon the character and amount of plan...

Within infected eukaryotic cells the two pathogenic Listeria species, L. monocytogenes and L. ivanovii, induce polymerization of cellular actin and the formation of a propulsive actin tail at one bacterial pole. For L. monocytogenes it has been shown that the product of the listerial actA gene is required for this process which is regarded as a model for actin-based motility. We have now cloned...