Adenosine 3’:5’-monophosphate (cyclic AMP)-dependent protein kinase which catalyzes the phosphorylation of histone and protamine is purified about loo-fold from the soluble fraction of bakers’ yeast by streptomycin treatment, ammonium sulfate fractionation, followed by DEAE-cellulose column chromatography and isoelectrofocusing electrophoresis. A divalent cation, Mg2+, Mn2+, or Co2+, is needed ...

Cabib and Leloir (2) isolated and characterized guanosine diphosphate (GDP) mannose from bakers’ yeast and postulated that this nucleotide may serve as a donor of mannose residues for the synthesis of polymers which are present in the yeast cell wall. The occurrence of GDP-mannose may be ubiquitous since it has been reported to be present in hen’s oviduct (3), the molds Penicillium chrysogenum ...

This study investigated the potential of oleaginous yeast Rhodotorula glutinis utilizing pulp and paper wastewater effluents as cultivation media for the sustainable production of microbial lipids as biodiesel feedstock. R. glutinis is oleaginous yeast, which has the ability to produce significant quantities of intercellular lipids in the form of triacylglycerols. Yeast lipids are a promising p...

The process of endogenous respiration of two strains of bakers' yeast, Saccharomyces cerevisiae, was examined kinetically. The rate of respiration with respect to time in a non-nutrient medium was found to exhibit two phases: (a) a period of constant rate of O(2) consumption and CO(2) production (R.Q. = 1) characteristic of cells with ample concentrations of stored material; (b) a first order d...

where k is the rate of the reaction, z is a constant, e is 2.718, R is the gas constant, T is the absolute temperature, and # represents the energy of activation in calories per tool of the pacemaker reaction in the catenary series of events controlling the rate (Crozier and Hoagland, 1934). Since most biological reactions are catalyzed by enzymes, the change in rate is determined by the enzyme...

For detailed studies on the mode of action of an enzyme a readily available source and a convenient method of preparation are of distinct advantage. Accessible sources such as bakers’ yeast (1) and spinach leaves (2) have, therefore, been used for large-scale preparations of transketolase in spite of the fact that extracts of Escherichia coli, in which the cleavage of ribose-5-P to triose-P was...

For detailed studies on the mode of action of an enzyme a readily available source and a convenient method of preparation are of distinct advantage. Accessible sources such as bakers’ yeast (1) and spinach leaves (2) have, therefore, been used for large-scale preparations of transketolase in spite of the fact that extracts of Escherichia coli, in which the cleavage of ribose-5-P to triose-P was...

Yeasts are an attractive group of lower eukaryotic microorganisms, some of which are used in several industrial processes that include brewing, baking and the production of a variety of biochemical compounds. More recently, yeasts have been developed as host organisms for the production of foreign (heterologous) proteins. Saccharomyces cerevisiae has usually been the yeast of choice, but an inc...

The starter sponge, or inoculum, used in the production of San Francisco sourdough French bread contains a unique heterofermentative Lactobacillus species that was first isolated by Kline and Sugihara (4). Based on the exacting nutritional requirements and genetic composition of this organism, these authors proposed the new species name Lactobacillus sanfrancisco for the sourdough bacterium. Su...

The protein releasing kinetics of Saccharomyces cerevisiae cells were investigated by using the ultrasonication method. The effects of acoustic power, duty cycle % of a sonicator, medium pH and cell concentration on protein release were examined. An attempt was also made to enhance cell disruption further by adding glass beads to the solution. An increase with protein release was observed with ...