Chargaff’s second parity rule (PR2) holds empirically for most types of DNA that along single strands of DNA the base contents are equal for complimentary bases, A = T,G = C. A Markov chain model is constructed to track the base evolution of any position along template strands of DNA whose replication is equipped with mismatch repair. Under the key assumptions that the mismatch error rates prim...

The two major lysine tRNAs from rat liver, tRNA2Lys and tRNA5Lys, were sequenced by rapid gel or chromatogram readout methods. The major tRNA2Lys differs from a minor form only by a base pair in positions 29 and 41; both tRNAs have an unidentified nucleotide, U**, in the third position of the anticodon. Although highly related, the major tRNA2Lys and tRNA5Lys differ in four base pairs and four ...

In this study, a single-base change discrimination strategy was developed through enhanced DNA toehold exchange reaction on a chip surface. A DNA strand with single-base change (SNPs, insertion or deletion) at an arbitrary position was clearly discriminated with nucleic acid concentrations from 0.8 nM to 1 μM through this method.

A catalytic Mannich reaction of a simple ester with no activating functionality at the α-position via a product-base mechanism was reported. The desired Mannich adducts were obtained in high yields using a catalytic amount of KH. This is a rare example of a Brønsted base-catalyzed Mannich reaction of unactivated esters as substrates.

We have reported a deficiency of a 91-kDa glycoprotein component of the phagocyte NADPH oxidase (gp91(phox)) in neutrophils, monocytes, and B lymphocytes of a patient with X chromosome-linked chronic granulomatous disease. Sequence analysis of his gp91(phox) gene revealed a single-base mutation (C --> T) at position -53. Electrophoresis mobility-shift assays showed that both PU.1 and hematopoie...

In the title mononuclear Cd(II) complex, [CdCl(2)(C(11)H(17)N(3))], the Cd(II) atom is coordinated by two Cl atoms and three N atoms from the tridentate Schiff base ligand in a distorted square-pyramidal environment. The three N atoms and one Cl atom constitute the base of the pyramid, whereas the other Cl atom occupies the apical position.

forces and moments which are generated at the base of the ma-nipulator. Hence, diiect application of the analysis and control schemes for the fixed base robots to space robots is not possible. Fig. 2. In the first category, the position and orientation of the + for the development of dynamic models of space ;bots.

The mitochondrial tyrosine tRNA from Neurospora crassa has been sequenced and found to have several interesting features: (i) It resembles prokaryotic rather than eukaryotic tyrosine tRNAs in that it possesses a large variable loop (loop III); moreover, it can be quantitatively aminoacylated by Escherichia coli tyrosyl-tRNA synthetase but not by yeast tyrosyl-tRNA synthetase. (ii) This tRNA dif...

The following nucleotide sequence (P450 HPH) has been cloned from cDNA library of adult human liver in Xgtll by immunoscreening method. We used polyclonal antibodies against cytochrome P-450 from phenobarbital-pretreated rats. The sequence is essentially identical to the sequences Hpl — 1 and M-12 described by (1) and (2) respectively for the P-450 IIC8 type gene. The sole difference in the tra...

The tRNA anticodon loops always comprise seven nucleotides and is involved in many recognition processes with proteins and RNA fragments. We have investigated the nature and the possible interactions between the first (32) and last (38) residues of the loop on the basis of the available sequences and crystal structures. The data demonstrate the conservation of a bifurcated hydrogen bond interac...