The integration of Microbial Fuel Cells (MFCs) in a microfluidic geometry can significantly enhance the power density of these cells, which would have more active bacteria per unit volume. Moreover, microfluidic MFCs can be operated in a continuous mode as opposed to the traditional batch-fed mode. Here we investigate the effect of fluid flow on the performance of microfluidic MFCs. The growth ...

We describe a set of novel, batch-mode algorithms we developed recently as one key component in scalable, deep neural network based speech recognition. The essence of these algorithms is to structure the singlehidden-layer neural network so that the upper-layer’s weights can be written as a deterministic function of the lower-layer’s weights. This structure is effectively exploited during train...

AIMS To make a quantitative evaluation of the differences in biofilm formation by Staphylococcus epidermidis using batch and fed-batch growth systems and to correlate this with production of the major biofilm polysaccharide, poly-N-acetyl glucosamine (PNAG). METHODS AND RESULTS Dry weight measurements of biofilms formed in batch and fed-batch conditions were compared with haemagglutination ti...

Segment models are a generalization of HMMs that can represent feature dynamics and/or correlation in time. In this work we develop the theory of Bayesian and maximum-likelihood adaptation for a segment model characterized by a polynomial mean trajectory. We show how adaptation parameters can be shared and adaptation detail can be controlled at run-time based on the amount of adaptation data av...

We present a simple, rapid procedure for reconstitution of Escherichia coli RNA polymerase holoenzyme (RNAP) from individual recombinant alpha, beta, beta', and sigma 70 subunits. Hexahistidine-tagged recombinant alpha subunit purified by batch-mode metal-ion-affinity chromatography is incubated with crude recombinant beta, beta', and sigma 70 subunits from inclusion bodies, and the resulting r...

The conditions for increased production of thermostable -amylase from Bacillus caldolyticus DSM 405 were investigated. Preliminary experiments in batch shake flasks led to an optimized initial cultivation medium. Shake flask experiments in extended-batch and in fed-batch mode of operation indicated that the -amylase production was enhanced by continuous feeding of starch. The activity of the -a...