An external force-assisted near-field illumination biosensor (EFA-NI biosensor) detects a target substance that is propelled through an evanescent field by an external force. The target substance is sandwiched between an antibody coupled to a magnetic bead and an antibody coupled to a polystyrene bead. The external force is supplied by a magnetic field. The magnetic bead propels the target subs...

Magnetic micro- and nanoparticles ('magnetic beads') have been used to advantage in many microfluidic devices for sensitive antigen (Ag) detection. Today, assays that use as read-out of the signal the number count of immobilized beads on a surface for quantification of a sample's analyte concentration have been among the most sensitive and have allowed protein detection lower than the fgmL(-1) ...

The quality of the weld joint is highly influenced by the welding parameters. Hence accurate prediction of weld bead parameters is highly essential to achieve good quality joint. This paper presents development of neural network models for predicting bead parameters such as depth of penetration, bead width and depth to width ratio for AISI 202 grade stainless steel GTAW plates. The use of this ...

For a commercially viable recombinant intracellular protein production process, efficient cell lysis and protein release is a major bottleneck. The recovery of recombinant protein, cholesterol oxidase (COD) was studied in a continuous bead milling process. A full factorial response surface methodology (RSM) design was employed and compared to artificial neural networks coupled with genetic algo...

Isolation and purification of biomolecules (doubleand singlestranded DNA, total RNAs, mRNAs, miRNA, proteins), as well as specific cells and organs, facilitate a wide array of downstream applications in gene and protein expression studies, cloning, transfection, protein-protein interactions, immunology, clinical diagnostics, cDNA library synthesis, PCR and qPCR, Sanger and NGS sequencing, and m...

Based on extensive Brownian dynamics simulations we study the thermally driven motion of a tracer bead in a cross-linked, dynamic gel network in the limit when the tracer bead’s size is of the same size or even larger than the equilibrium mesh size of the gel. The analysis of long individual trajectories of the tracer bead demonstrates the existence of pronounced transient anomalous diffusion, ...

The influence of the bead-bead interaction on the rotational dynamics of macromolecules which are immersed into a solution has been investigated by starting from the microscopic theory of the macromolecular motion, i.e., from a Fokker-Planck equation for the phase-space distribution function. From this equation, we then derived an explicit expression for the configuration-space distribution fun...

Proper fusion is crucial in generating a sound weld. Successful control of the fusion state requires accurate measurements of both the top-side and back-side bead widths. A top-side sensor based system is preferred so that the sensor can be attached to and moved with the torch. Thus, the system must be capable of estimating the back-side bead width with high accuracy. Because skilled human oper...

VASP comet sightings ess is more when it comes to actin-fueled propulsion. Julie Plastino, Stéphane Olivier, and Cécile Sykes (Institut Curie, Paris, France) show that hollow actin comets propel beads faster than a more filament-packed comet. Actin comets, which drive bead or bacteria movement, are thought to depend strongly on Arp2/3–built, branched actin networks. Now, Plastino et al. show th...

The effect of process parameters on the forming profile nickel-based alloy deposited by plasma and cold metal transfer (CMT) wire-arc deposition was comparatively studied. arc welding (PAW) presented good wettability high dilution rate, while CMT showed opposite. ellipse parabola function models for single weld bead were established MATLAB software. results that model had higher similarity with...