The HMG-box domain of the human male sex-determining factor SRY, hSRY(HMG) (comprising residues 57-140 of the full-length sequence), binds DNA sequence-specifically in the minor groove, resulting in substantial DNA bending. The majority of point mutations resulting in 46X,Y sex reversal are located within this domain. One clinical de novo mutation, M64I in the full-length hSRY sequence, which c...

A combination of simulations and Fourier transform infrared spectroscopy was used to examine the effect of three ionic solutes ~KCl, NaCl, and KSCN!, the polar solute urea, and the osmolyte trimethylamine-N-oxide ~TMAO! on a water structure. The ionic solutes increase the mean water– water H-bond angle in their first hydration shell concomitantly shifting the OH stretching mode to higher freque...

Bacteriophage phi29 protein p4 is essential for the regulation of the switch from early to late phage transcription. The protein binds to two regions of the phage genome located between the regulated promoters. Each region contains two inverted repeats separated by 1 bp. We used circular permutation assays to study the topology of the DNA upon binding of the protein and found that p4 induced th...

An EPG-representation of a graph G is a collection of paths in a plane square grid, each corresponding to a single vertex of G, so that two vertices are adjacent if and only if their corresponding paths share infinitely many points. In this paper we focus on graphs admitting EPG-representations by paths with at most 2 bends. We show hardness of the recognition problem for this class of graphs, ...

We report on some extensions of the Kandinsky model: A new and highly nontrivial technique to incorporate nonplanar drawings into the Kandinsky model in the same way as in the GIOTTO approach is presented. This means a major step towards the practical usability of our approach. The used technique even gives new insights for the solvability of network ow problems. Another variant of Kandinsky en...

The current study used video clips of bowing actions depicted by three-dimensional computer graphics. The bend angle (15 degrees and 45 degrees) and duration of the bent posture (0-4.5 seconds) were varied. In the first experiment, the participants rated their subjective impressions of the bowing actions. The bowing actions that were made at a 45 degrees angle and held for more than 1 second we...

Accurate prediction of the final shape using numerical modeling has been a top priority in the field of sheet and bulk forming. Better shape prediction is the result of a better estimation of the physical stress and strain state. For experimental and numerical investigations of such estimations, simple benchmark processes are used. In this paper a benchmark process involving forging (flattening...

Wind turbines face harsh inflow conditions when operating in the atmospheric boundary layer or wake of other wind turbines. The incoming velocity field can change within seconds due to turbulent structures it contains, resulting a rapid several degrees angle attack for rotating blade. Aerodynamics are hence rapidly altered, leading changes occurring forces on rotor. Such dynamic cause blades tw...