Weanling rats were fed a simple riboflavin-deficient diet or the same diet supplemented with galactoflavin. Oxidative phosphorylation was studied in isolated hepatic mitochondria. Throughout the course of the experimental diets, a total of 8 weeks, there were no alterations in the respiratory control ratios or in the ADP/O ratios of the isolated mitochondria. Succinate dehydrogenase activity de...

The development of fresh meat quality is dictated by biochemical changes during the perimortem and postmortem period. Lipid protein oxidation in skeletal muscle products detrimental to product quality. mechanisms that influence lipid remain unelucidated. Peroxiredoxins are thiol-specific antioxidant proteins highly reactive abundant may be involved limiting early postmortem. This review aims pr...

Several years ago we reported that formaldehyde could be isolated when dimethylaminoethanol, dimethylglycine, sarcosine, or methanol was incubated with a whole liver homogenate (1). Dimethylaminoethanol differed from the other substrates in that it required both the supernatant and sediment fractions of the homogenate to effect this biochemical oxidation. We have now prepared methyl-labeled dim...

The recently cloned and characterized human polyamine oxidase (PAOh1) potentially represents a new class of catabolic enzymes in the mammalian polyamine metabolic pathway capable of the efficient oxidation of polyamines. Here the discovery of three additional human PAO splice variants is reported, and the data support the fact that the human PAO gene codes for at least four isoenzymes, each of ...

Increased blood plasma concentrations of the sulphur amino acid homocysteine ("homocysteinemia") have been brought into context with neurodegenerative and arteriosclerotic symptoms and diseases. We recently reported on biochemical model reactions on the prooxidative activity of homocysteine including the desactivation of Na+/K(+)-ATPases and hemolysis of erythrocytes (Preibisch et al., 1993). I...

Nanoporous silicon optical waveguides, which serve as the basic foundation for porous silicon based biochemical sensors, have been fabricated by electrochemical etching and laser local oxidation. By optimizing the porous silicon layers, laser writing power and speed, acceptable optical losses have been achieved. Loss mechanisms include scattering, intrinsic absorption, coupling loss at the fibe...

The objective of the research was to study signs oxidative stress, state proteolysis, and proteinase-inhibiting system blood plasma in rats with streptozotocin-induced diabetes mellitus (60 mg/kg intra-abdominally), complicated by cerebral ischemia-reperfusion. Levels products lipid peroxide oxidation, protein modification, nitrogen oxide metabolites, activity antioxidant protection enzymes wer...

Human thymine DNA glycosylase (hTDG) efficiently excises 5-carboxylcytosine (5caC), a key oxidation product of 5-methylcytosine in genomic DNA, in a recently discovered cytosine demethylation pathway. We present here the crystal structures of the hTDG catalytic domain in complex with duplex DNA containing either 5caC or a fluorinated analog. These structures, together with biochemical and compu...

A family of acyl-CoA oxidase isozymes catalyse the first step in the peroxisomal fatty acid beta-oxidation spiral. Our group and others have recently characterized four genes from this family in the model oilseed Arabidopsis. These genes encode isozymes with different acyl-CoA substrate specificities, which together encompass the full range of fatty acid chain lengths that exist in vivo. Here w...

In the view of constantly increasing number of reports about participation of advanced oxidation protein products (AOPPs) in pathogenesis of many diseases as well as in the pathomechanism of their biochemical disturbances and clinical complications, the possibility of treatment of these diseases by reducing of AOPPs concentration and prevention or inhibition of their formation poses the object ...