In this study, we describe the distribution of various classes of proteoglycans and their potential matrix ligand, hyaluronan, during neural crest development in the trunk region of the chicken embryo. Different types of chondroitin and keratan sulfate proteoglycans were recognized using a panel of monoclonal antibodies produced against specific epitopes on their glycosaminoglycan chains. A hep...

The effect of ascorbic acid (AA) and butylated hydroxyanisole (BHA) supplementation on aflatoxicosis in broiler chickens (1 to 42 days of age) was investigated (250, 500, 1000 and 2000 ppm for AA and BHA) in diet containing 1 ppm total aflatoxin (AF: 76.45% AFB1, 10.52% AFB2, 9.89% AFG1 and 3.14% AFG2). A total of 300 day-old broiler chicks were divided into ten treatment groups (T1=control; T2...

Purified total genomic DNA labeled with photobiotin was used as a probe for the identification and differentiation of strains of Echinococcus granulosus and to distinguish between the morphologically identical eggs of 2 taeniid species. Such biotinylated probes are easily prepared and can be utilized effectively for rapid screening and identification of taeniid isolates by dot and Southern blot...

Job KM, Dull RO, Hlady V. Use of reflectance interference contrast microscopy to characterize the endothelial glycocalyx stiffness. Am J Physiol Lung Cell Mol Physiol 302: L1242–L1249, 2012. First published April 13, 2012; doi:10.1152/ajplung.00341.2011.— Reflectance interference contrast microscopy (RICM) was used to study the mechanics of the endothelial glycocalyx. This technique tracks the ...

Several studies have suggested that chondrocytes must have the capacity to internalize and degrade extracellular hyaluronan. In the present study we show direct evidence that hyaluronan is, in fact, endocytosed by chondrocytes and that the endocytosis is mediated via cell surface CD44/hyaluronan receptors. Cultures of bovine articular chondrocytes as well as rat chondrosarcoma chondrocytes were...

Chemiluminescent detection of polymerase chain reaction (PCR-)amplified DNA was used as a quantitative method for detecting HIV-1. For this purpose, biotinylated dUMP was directly incorporated into the amplified DNA during the PCR reaction. Biotinylation was visualized in an enzymatic reaction using avidine-conjugated alkaline phosphatase and its chemiluminescent 1,2-dioxetane substrate AMPPD, ...

Yb-doped TiO₂ (Yb/TiO₂) compositions were synthesized by sol-gel method, and the prepared materials were characterized by X-ray Diffraction (XRD), X-ray photoelectron spectroscopy (XPS), UV-visible diffuse-reflectance spectrum (UV-Vis DRS), transmission electron microscope (TEM) and high resolution transmission electron microscope (HRTEM), energy dispersive spectrometer (EDS), and N₂ adsorption...

In this study we tested the genotoxicity of two widely used phenolic antioxidants, butylated hydroxyanisole (BHA) and tert-butylhydroquinone (TBHQ) in multiple mouse organs using the alkaline comet assay. Tissue samples from four organs (stomach, liver, kidney and bone marrow) were collected from male mice at 3 and 24 h post treatment with BHA (800 mg/kg) or TBHQ (400 mg/kg) and examined for ge...

When administered prior to or at the time of carcinogen exposure, the phenolic antioxidants butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) are effective inhibitors of carcinogenesis in several target organs. However, chronic, postcarcinogen administration of BHT apparently enhances tumorigenesis in certain animal models for liver and lung cancer. The present study was perform...