UNLABELLED Many time-consuming analyses of next -: generation sequencing data can be addressed with modern cloud computing. The Apache Hadoop-based solutions have become popular in genomics BECAUSE OF: their scalability in a cloud infrastructure. So far, most of these tools have been used for batch data processing rather than interactive data querying. The SparkSeq software has been created to ...

5.1 DNA Sequence Determination 168 5.1.1 Principles of DNA Sequencing 168 5.1.2 Automated Fluorescent DNA Sequencing 169 5.1.3 RNA Sequencing by Reverse Transcription 170 5.2 Gene Cloning 170 5.2.1 Classical Cloning 170 5.2.2 The Polymerase Chain Reaction 173 5.3 Enzymes Useful in Gene Manipulation 174 5.3.1 Restriction Endonucleases 174 5.3.2 Other Nucleases 175 5.3.3 Polynucleotide Kinase 176...

In this article we discuss a strategy for speeding up the solution of the NavierStokes equations on highly complex solution domains such as complete aircraft, spacecraft, or turbomachinery equipment. We have used a nite-volume code for the (non-turbulent) Navier-Stokes equations as a testbed for implementation of linked numerical and parallel processing techniques. Speedup is achieved by the Ta...

clostridium septicum a gram positive anaerobic bacterium produces several toxins including alpha, beta, gamma and delta. c. septicum alpha toxin is lethal and is responsible for a serious disease known as gas gangrene. the aim of the present study was to molecular cloning and sequencing of c. septicum vaccine strain alpha toxin gene. genomic dna was extracted using standard phenol and chlorofor...

background: multiple sclerosis (ms), a chronic inflammatory demyelinating disorder with neurodegenerative aspects, is more common among young adults, particularly women. methods: this molecular study was designed to investigate the il-7r α chain gene in iranian ms patients. we studied 60 ms patients, diagnosed based on 2005 r-mcdonald criteria and 60 apparently healthy individuals as the contro...

the aim of current study was to investigate the nucleotide sequence of seven trna genes in mitochondrial gene of khorasan’s native chicken and identification of possible mutations in it. for performing it, this part of the gene was cloned using the specific amplification initiators and cloned in the plasmid ptg19-t vector in dh5α strain of e-coli after the sampling and extracting the dna from w...

background: we examined a molecular method with a single-pcr for amplification of a part of cp5 gene enabling us to differen­tiate the pathogenic species, entamoeba histolytica , from the non-pathogenic species, e. dispar . methods: we developed a single pcr method for this purpose.  after investigation of genbank, primer pairs were de­signed from highly conserved regions of cysteine proteinase...