BACKGROUND Different serological tests are used in serologic diagnosis of brucellosis. The most widely used of these are Standard Tube Agglutination and Coombs anti-brucella tests. Whereas ELISA Ig M and Ig G tests have been in use for a long time, immuncapture agglutination test has been recently introduced and used in serological diagnosis. The aim of this study was to compare diagnostic valu...

An indirect ELISA test was developed as a novel tool aimed at monitoring the herd infection status of swine herds. Meat juice samples from pig carcasses were analysed for the presence of antibodies against porcine reproductive and respiratory syndrome virus (PRRSV). A study of samples from herds with known PRRS status was undertaken. The PRRS status of the herds was evaluated based on the analy...

1. Abstract 2. Introduction 3. Materials and methods 3.1. Clinical Characteristics of Patients 3.2. Serum Collection 3.3. Tumor Cyst Fluid Collection 3.4. ELISA for TNF and stnf-r’s 3.5. Cytolytic Bioassay for TNF Activity and TNF Blocking Factor Activity 3.6. Immunohistochemical Staining of Astrocytoma Tissue Sample 3.7. Statistical Analysis 4. Results 4.1. Serum Levels of TNF and the 55 kda a...

La diarrea viral bovina (DVB) es una patología infecciosa generada por un pestivirus de distribución mundial, causante problemas reproductivos y pérdidas económicas. El objetivo del presente estudio fue establecer la positividad al virus (vDVB) en vacas municipio Tuta (Boyacá, Colombia), analizar los grupos etarios, raciales las variables reproductivas manejo como posibles factores riesgo. Se t...

An indirect enzyme-linked immunosorbent assay (I-ELISA) based on the recombinant VP3 protein of duck hepatitis A virus type 1 (DHAV-1) was developed and evaluated in this study. The optimal antigen, serum and enzyme-labeled antibody dilutions were 1:160 (0.94μg), 1:160 and 1:2000, respectively. The optimal blocking buffer was 1% gelatin. The cutoff value was determined to be 0.332, and the anal...

In the biomedical field, end-point detection bioassays such as enzyme-linked immunosorbent assays (ELISAs) are essential tools because of their simplicity, high-throughput, and suitability for their use at the point-of-care. End-point bioassays are significantly constrained by the need of sample labeling with fluorescent or colorimetric tags for subsequent detection. A promising strategy to ove...

BACKGROUND Monckeberg's calcification in diabetes, known as medial artery calcification, is an independent predictor of cardiovascular mortality. However, the mechanism underlying this phenomenon remains to be elucidated. We demonstrate that advanced glycation end products (AGEs) induce calcification of vascular smooth muscle cells through the receptor for AGE (RAGE)/p38 mitogen-activated prote...

Traditional geostatistical space-time geostatistics is not able to take account of the generally strongly non-linear dynamics of multivariate space-time processes. To this effect physico-chemical transport models are in general more suitable. However, as the latter do not fully master the complexity of the processes they attempt to describe, either because of simplifying hypotheses or because t...

In this paper, we present new attacks on the redactable signature scheme introduced by Kundu and Bertino at VLDB ’08. This extends the work done by Brzuska et al. at ACNS ’10 and Samelin et al. at ISPEC ’12. The attacks address unforgeability, transparency and privacy. Based on the ideas of Kundu and Bertino, we introduce a new provably secure construction. The corresponding security model is m...