Two hundred and thirty-nine cattle from Gauteng Province in South Africa were tested for various pathogens causing reproductive diseases includingbovine viral diarrhoea/mucosal disease (BVD/MD) virus, infectious bovine rhinotracheitis/infectious pustular vulvovaginitis (IBR/IPV) virus, Neospora caninum and Brucella abortus usingvarious tests. For BVD/MD virus, 49.37% tested positive, 74.47% for...

To assess sources of variation in nuclear transfer efficiency, bovine fetal fibroblasts (BFF), harvested from six Jersey fetuses, were cultured under various conditions. After transfection, frozen-thawed lung or muscle BFF donor cells were initially cultured in DMEM in 5% CO(2) and air and some were transferred to MEM, with 5% or 20% O(2) or 0.5% or 10% serum and G418 for 2-3 wk. Selected clona...

Gastrin-releasing peptide (GRP) has been suggested as a novel regulatory peptide in the female reproductive tract but the presence of GRP and GRP mRNA in the non-neurogenic tissue of the cervix has not yet been clarified. In the present study, immunohistochemistry and in situ hybridization were used to reveal the distribution of GRP immunoreactivity and expression of GRP mRNA in the bovine cerv...

of the known mycotoxins, the most important in relation to direct hazard to human health are the aflatoxins produced by a large number of aspergillus spp. to determine the level of exposure of aflatoxin from mother to child and its mode of transfer. 70 pregnant women in the labor ward of the federal medical centre yola were investigated for their aflatoxin content by using the velasco fluorotox...

BACKGROUND A survey was conducted to determine the prevalence of campylobacteriosis and trichomonosis, and their concurrence with brucellosis, in cattle in three states of northern Nigeria. METHODS A total of 602 preputial samples was collected from bulls in 250 herds and tested using culture and identification. Various indigenous and exotic breeds were studied and four major management syste...

Microaerophilic, motile, gram-negative, comma-like bacteria have been found associated with the genital organs of the ovine and bovine species. These curved rods are believed to be an important cause of sterility in cows and sheep. Smith and Taylor (1919) proposed the name Vibrio fetus for these microorganisms. Recently, Brynei and Frank (1955) and Reich et al. (1956) found, using catalase and ...

According to the classification of placental types among animals, the transfer of iron through the placenta can occur via: absorption connected to transferin through the outer surface of the trophoblast in direct contact with circulating maternal blood; absorption of the erythrocytes by the chorionic epithelium in direct contact with accumulation of blood extravased from haemotophagous areas; a...