The polymerase chain reaction (PCR) is used widely for the in vitro amplification of DNA. The versatility of PCR can be enhanced by the introduction of sequential steps such as nested primer amplifications (1) or re-amplification protocols (2). PCR can also be done on cDNA templates produced by reverse transcription of RNA (3). These protocols require additional opening and closing of the react...

1. A soluble reduced Methyl Viologen-dependent assimilatory nitrate reductase from Azotobacter vinelandii strain UW136 grown aerobically on nitrate was purified to homogeneity by the criteria of nitrate reductase activity staining, and coincidence of a Coomassie Blue-staining protein band on polyacrylamide gels run under non-denaturing conditions. The specific activity was 3 mumol of NO2- forme...

The present study was conducted on eighteen male Assam goats ranging in age from day old to 10 months divided into six post natal age groups viz. group-I (0-day), group-II (2 months), group-III (4 months), group-IV (6 months), group-V (8 months) and group-VI (10 months) consisting of three animals in each group. Tissue pieces were collected from cauda epididymides and subsequently fixed in Boui...

bromophenol is formed from bromobenzene-3,4-oxide nonenzymically, the decrease in p-bromophenol formation may be used as an indirect measure of the decrease in the extracellular concentration of bromobenzene-3,4-oxide in the presence of the transferase. From these data, we found that only negligible amounts of the epoxide re-entered the cells; that is, kZ1 was negligible and that about 59% of t...

Predictable behaviour is a critical factor when developing a sensor for potential deployment within a wireless sensor network (WSN). The work presented here details the fabrication and performance of an optical chemical sensor for gaseous acetic acid analysis, which was constructed using inkjet printed deposition of a colorimetric chemical sensor. The chemical sensor comprised a pH indicator dy...

A spectrophotometric method for the determination of ionic surfactants with Bromophenol Blue (BPB) based on incorporation into a precipitated chitosan was studied. Cationic surfactants (CS+), such as a quaternary ammonium ion containing a long-chain alkyl group, associate with BPB2- buffered at about pH 9 to form the ion associate (CS+)2 x BPB2-. CS+ associates with anionic surfactants (AS-). I...

Ablood purification module was developed with edible agar or Gelrite, in which activated charcoal was dispersed. Tap water (or normal saline) was boiled, and agar (or Gelrite) powder was dissolved in it. The solution was hardened with no additive or activated charcoal in a plastic cylindrical hard shell with a perforated bottom plate to assemble module A (agar only), module AC (agar with variou...

Two alpha1-globulin bands of fetal serum with relative mobilities against bromophenol blue of 0.55 and 0.58 on 7% polyacrylamide gel electrophoresis reacted with a monospecific rabbit antiserum to alpha-fetoprotein (AFP). The former globulin band was clearly detected in the fetal liver supernatant. AFP was immunochemically purified from both the fetal serum and liver, and their electrophoretic ...