Tigecycline is a broad spectrum antibiotic having activity against multiresistant isolates. In vitro susceptibility testing is difficult to perform with the use of traditional microbiological techniques. The aim of this study was to evaluate the disk diffusion test with three different Mueller-Hinton agar brands, and the Vitek 2 automated system in comparison with the standard broth microdiluti...

Essential oils of Magnolia balansae A. DC. growing wild in Son La (leaf, twig and fruit) Phu Tho (fruit) provinces Vietnam were obtained by hydrodistillation analyzed using GC/MS-FID. From 30 to 49 compounds identified accounting for 66.1–80.0% the oils. Spathulenol (33.9%, 20.7%, 11.2%), caryophyllene oxide (10.0%, 10.1%, 9.3%) main components leaf, fruit M. balansae, respectively, from provin...

Antimicrobial photodynamic inactivation is considered a promising antimicrobial approach that may not develop resistance in the near future. Here, we investigate influence of photosensitizer chlorophyllin (CHL) and cationic permeabilizer polyethylenimine (PEI), exposed to red light-emitting diode, on human pathogen Pseudomonas aeruginosa free-living planktonic cells, sessile biofilm persister c...

Haemophilus test medium (HTM) was developed recently for dilution and disk diffusion antimicrobial agent susceptibility testing of Haemophilus influenzae. The application of HTM to the testing of other, less frequently encountered Haemophilus species recovered from humans was evaluated in this study by using commercially prepared HTM (BBL Microbiology Systems, Cockeysville, Md.) in broth microd...

Since the early 1970s, the synergistic activity of an aminoglycoside with a cell wall-active agent has been predicted by determining the ability of an enterococcus to grow in the presence of high levels of the aminoglycoside (usually > or = 2,000 micrograms/ml). However, a variety of media and concentrations of aminoglycosides has been used for this screening procedure. In the present study, we...

A recent study reporting surprisingly high beta-lactam MICs against beta-lactamase-negative ampicillin-resistant (BLNAR) strains when tested by the Etest prompted us to compare results of the Etest with those of the standard broth microdilution concerning BLNAR strains (5). In that study, the MIC90s of BLNAR and beta-lactamase-positive amoxicillinclavulanate-resistant (BLPACR) strains in respon...

Seven commonly used antimicrobial susceptibility testing methods were used to test the susceptibility of 150 isolates of Pseudomonas aeruginosa against gentamicin, tobramycin, amikacin, carbenicillin, and piperacillin. Results were compared with respect to the susceptibility characteristics of the population of isolates as defined by each method. Conventional methods included agar disk diffusio...