Forward genetic analysis is one of the principal advantages of the zebrafish model system. However, managing zebrafish mutant lines derived from mutagenesis screens and mapping the corresponding mutations and integrating them into the larger collection of mutations remain arduous tasks. To simplify and focus these endeavors, we developed an approach that facilitates the rapid mapping of new zeb...

A natural male sterile mutant of Salvia miltiorrhiza (Labiatae, Sh-B) was found during field survey in 2002. Our objective was to analyze its genetic mechanism for producing F1 hybrid seeds and to develop a molecular marker linked to male sterile gene for selection of a hybrid parent line. The segregation ratios of male sterile plants to fertile plants in the progenies of both testcross and bac...

Wilt, caused by Fusarium oxysporum f. sp. ricini, is the most severe disease of castor bean (Ricinus communis). For the development of marker for wilt-resistant gene, 200 F2 individuals from a cross between the resistant cultivar 48-1 and the susceptible cultivar VP-1 were inoculated with a race of Fusarium oxysporum f. sp. ricini. Bulked segregant analysis was used to search for random amplifi...

Consistent grain yield in drought environment has attracted wide attention due to global climate change. However, the important drought-related traits/genes in crops have been rarely reported. Many near-isogenic lines (NILs) of male sterile and fertile Salvia miltiorrhiza have been obtained in our previous work through testcross and backcross in continuous field experiments conducted in 2006-20...

An F(1) mapping population of the septoria tritici blotch pathogen of wheat, Mycosphaerella graminicola, was generated by crossing the two Dutch field isolates IPO323 and IPO94269. AFLP and RAPD marker data sets were combined to produce a high-density genetic linkage map. The final map contained 223 AFLP and 57 RAPD markers, plus the biological traits mating type and avirulence, in 23 linkage g...

doi: 10.1111/j.1399-3054.2006.00739.x To investigate selenium tolerance mechanisms in Arabidopsis thaliana, genetic and physiologic studies were performed in the three Arabidopsis accessions Landsberg erecta (Ler), Columbia (Col) and Wassilewskija (Ws). Accession Ler was significantly less tolerant to selenate than Ws and Col, whereas Ws was less tolerant to selenite than the others. Analysis o...

Next-generation sequencing (NGS)-based bulked-segregant analysis (BSA) approaches have been proven successful for rapidly mapping genes in plant species. However, most such methods are based on mutants and usually only one gene controlling the mutant phenotype is identified. In this study, NGS-based BSA was employed to map simultaneously two qualitative genes controlling cotyledon color of seed...

Cytoplasmic male-sterility (CMS) has been exclusively used to produce F1 hybrid seeds of onion (Allium cepa L.). A single nuclear locus, Ms, is known restore male-fertility CMS in onions. Unstable male-sterile onions producing a small amount pollen grains have identified previous study. When such unstable were crossed with stable containing CMS-R cytoplasm, was completely restored, although gen...

Spike length (SL) and spike compactness (SC) are crucial traits related to wheat ( Triticum aestivum L.) yield potential. In this study, a backcrossed inbred lines (BILs) population segregating for SL/SC was developed by using commercial variety chuanyu25 as recurrent parent backbone Chuanyu12D7. Bulked segregant analysis (BSA) combined with the Wheat 660K SNP array performed conduct quantitati...

Low temperature is a major limiting factor in rice growth and development. Mapping of quantitative trait loci (QTLs) controlling cold tolerance is important for rice breeding. Recent studies have suggested that bulked segregant analysis (BSA) combined with next-generation sequencing (NGS) can be an efficient and cost-effective way for QTL mapping. In this study, we employed NGS-assisted BSA to ...