A practically simple top-down process for the exfoliation of graphene (GN) and few-layer graphene (FLG) from graphite is described. We have discovered that a biocompatible amphiphilic pyrene-based hexahistidine peptide is able to exfoliate, functionalize, and dissolve few layer graphene flakes in pure water under exceptionally mild, sustainable and virtually innocuous low intensity cavitation c...

Many proteins that accumulate in the form of insoluble aggregates when they are overproduced in Escherichia coli can be rendered soluble by fusing them to E. coli maltose binding protein (MBP), and this will often enable them to fold in to their biologically active conformations. Yet, although it is an excellent solubility enhancer, MBP is not a particularly good affinity tag for protein purifi...

Y. H. BRENNER (1881-1921) has long been associated with the great Russian writers of the nineteenth century. His early letters express fascination with Russian novelists, poets, and critics (Brenner, 1967, pp. 218-226), and the figures of Tolstoy and Dostoyevsky appear many times in both his fiction and non-fiction writing. Brenner's admiration for the depth of Dostoyevsky's psychological portr...

In her article "Human Cloning as the Other in Ishiguro's Never Let Me Go" Wen Guo analyzes Kazuo Ishiguro's novel with focus on Ishiguro's analogy between human cloning and people of marginality in contemporary society. Guo discusses the novel's ambience of doubt and suspense and elaborates on how the theme of otherness is addressed by Ishiguro's mock-realism in a landscape of science fiction. ...

A surface plasmon resonance imaging-based Ni(2+)-iminodiacetic acid-coated gold chip system was developed to enable specific detection of a hexahistidine-tagged recombinant protein in crude extracts or in column chromatography fractions. This system is especially advantageous for high-throughput analysis of multiple proteins.

Fluorescent probes are essential for the exploration of protein function, detection of molecular interactions, and conformational changes. The nitrilotriacetic acid derivatives of different chromophores were successfully used for site-selective noncovalent fluorescence labeling of histidine-tagged proteins. All of them, however, suffer from the same drawback--loss of the fluorescence upon bindi...

Affinity tags have become powerful tools from basic biological research to structural and functional proteomics. They were widely used to facilitate the purification and detection of proteins of interest, as well as the separation of protein complexes. Here, we mainly discuss the benefits and drawbacks of several affinity or epitope tags frequently used, including hexahistidine tag, FLAG tag, S...

Urdu novel has very important in a literature. It its own historical perspectives. Abdullah Hussain was renowned fiction writer Urdu. He is considered trend and written style setter writers. In this essay his new unique of modern being discussed the perspective fiction. also analysed role to create new, mix novels. Hussain’s Novel have big complex, weave many strands, violent viability, like st...

The aim of this study is to discuss Murakami Haruki’s novel Killing Commandatore (Kishidanchou Goroshi) in terms transtextuality and analyze the allusions intertextual references Murakami’s narrative. This argues Commendatore adopting Gennette’s framework his theory metalepsis as well utilizing term “mise en abyme” which was characterized by André Gide. has considerable amount relationships wit...