BACKGROUND Despite intensive-alkylator based regimens, >50% of patients with high-risk neuroblastoma (NB) die from recurrent disease that is probably due, in part, to acquired alkylator resistance. PROCEDURE Using buthionine sulfoximine (BSO)-mediated, glutathione (GSH) depletion to modulate melphalan (L-PAM) resistance, we examined six NB cell lines established after progressive disease foll...

BACKGROUND & AIMS Acquisition of anoikis resistance is a prerequisite for metastasis in hepatocellular carcinoma (HCC). However, little is known about how energy metabolism and antioxidant systems are altered in anoikis-resistant (AR) HCC cells. We evaluated anti-tumor effects of a combination treatment of 3-bromopyruvate (3-BP) and buthionine sulfoximine (BSO) in AR HCC cells. METHODS We com...

The purpose of this study was to examine the effects of glutathione (GSH) depletion and cellular oxidation on rat diaphragm contractility and sarco(endo)plasmic reticulum Ca(2+)-ATPase (SERCA) function in vitro under basal conditions and following fatiguing stimulation. Buthionine sulfoximine (BSO) treatment (n = 10) for 10 days (20 mM in drinking water) reduced (P < 0.05) diaphragm GSH content...

Glutathione (GSH) is an important factor involved in the resistance of tumor cells to anticancer agents. Buthionine sulfoximine (BSO), a specific inhibitor of GSH synthesis, effectively decreases cellular GSH concentrations both in vitro and in vivo. Depletion of GSH by BSO sensitizes a variety of cancer cells to chemotherapeutic agents. Therefore, BSO has been on clinical trial as an anticance...

Time integration' has proved to be a powerful technique in optical signal processing and has been used in a wide variety of architectures. A major drawback of time-integrating processors is the buildup of bias in addition to the signal. This occurs because the photogenerated charge that is integrated on the detector is proportional to the intensity of the optical signal which makes it necessary...

Abstract: This paper studies the development and decay of surface discharge under various voltage waveforms and electrode shapes in air at atmospheric pressure. The electro-optical Pockels method is utilised to measure and quantify the distribution of surface charge. Charge is deposited on the surface of the Bismuth Silicon Oxide (BSO) sensing crystal from a high voltage needle or mushroom elec...

When the yeast Saccharomyces cerevisiae sigma 1278b was starved for nitrogen, the total glutathione (GSH) pool increased from 7 to 17 nmol (mg dry wt)-1 during the first 2 h and then declined. More than 90% of the total GSH shifted towards the central vacuole during this time. This transient stimulation was not observed in the presence of buthionine-(S,R)-sulphoximine (BSO), a specific transiti...

Excess numbers of X-ray sources around bright Seyfert galaxies have been demonstrated with significances of association up to 7.4 sigma (Radecke, preceding paper). The optical identification of these sources is shown here to be predominantly blue stellar objects (BSO’s) of which some are already catalogued as quasars. Excluding the two brightest Seyferts, a subset of 24 with apparent magnitudes...