The response of an extremely thermoacidophilic archaeon, Metallosphaera sedula (growth temperature range, 50 to 79(deg)C; optimum temperature, 74(deg)C; optimum pH, 2.0), to thermal stress was investigated by using a 10-liter continuous cultivation system. M. sedula, growing at 74(deg)C, pH 2.0, and a dilution rate of 0.04 hr(sup-1), was subjected to both abrupt and gradual temperature shifts i...

The magnitude of demand for planting materials in grape, mainly for rootstock genotypes indicates that micropropagation is inevitably necessary for their mass scale propagation. Therefore, the studies on micropropagation of four genetically different grape rootstocks namely Dogridge (Vitis champini), SO4 (V. riparia× V. berlandieri), H-144 (V. vinifera × V. labrusca) and 3309 C (V. riparia × V....

The present study is concerned with the production of cephalosporin C by Cephalosporium acremonium C-10. Two culture media were tested for the production of antibiotic. Among these culture media M1 containing sucrose, ammonium acetate, cornstarch, sugar cane molasses, soybean meal, calcium carbonate, calcium sulphate and methyl oleate gave optimum production of cephalosporin C. The cultural con...

A new rapamycin producing culture was isolated and designated as Actinoplanes sp. N902-109. The addition of a cytochrome P-450 inhibitor and precursor feeding using this culture, and biotransformation approaches generated new rapamycin analogs with modifications at C-4, C-9, C-16, C-27, C-28 and/or C-39. The immunosuppressive activity of the resulting analogs was established in the mixed lympho...

The receptor tyrosine c-Kit and its cognate ligand, c-Kit ligand (KL, stem cell factor, SCF), are involved in ovarian follicular development in several animal species. We studied the expression of KL and c-Kit using in situ hybridization and immunohistochemistry in donated human ovarian cortical tissue. The KL transcripts were expressed in granulosa cells of primary follicles, whereas the expre...

One of the primary goals antiviral research in recent decades has been biosynthesis new agents. The large number bioactive agents extracted from microbial isolates led to building connections between products through screening. Using a genotype 4 plasmid, extract fraction Pseudomonas oleovorans evaluated against HCV Hepatitis C cell culture. cytotoxicity assay using MTT [3-(4, 5-dimethylthiazol...

Campylobacter jejuni, one of the most common causes of human gastroenteritis, is a thermophilic and microaerophilic bacterium. These characteristics make it a fastidious organism, which limits its ability to survive outside animal hosts. Nevertheless, C. jejuni can be transmitted to both humans and animals via environmental pathways, especially through contaminated water. Biofilms may play a cr...

conclusions the results of our study showed that non-albicans species of candida are more frequent than c. albicans in patients with vvc. this result is in line with some recent studies indicating that non-albicans species of candida must be considered in gynecology clinics due to the reported azole resistance in these species. results out of 234 patients with vulvovaginitis, 66 (28.2%) patient...

An amplification polymerase chain reaction (PCR) test for the direct detection of Chlamydia trachomatis in urethral and endocervical swab specimens from symptomatic and asymptomatic women and men were compared to standard culture technique. During 6 months, 300 endocervical swab specimens from 205 asymptomatic women (64.4%) and 95 symptomatic women (31.6%), and 187 urethral swab specime...