In this study, we demonstrate potent regulatory function of the murine killer cell inhibitory receptor-like molecules, paired Ig-like receptors (PIRs) or p91, using chimeric receptors expressed on the rat basophilic leukemia cell line RBL-2H3. One of the chimeras, which has the transmembrane and cytoplasmic domain of PIR-B fused to the extracellular portion of type IIB receptor for IgG, was abl...

The Ca2+-sensing receptor (CaR) is a pleiotropic, type III G protein-coupled receptor (GPCR) that associates functionally with the cytoskeletal protein filamin. To investigate the effect of CaR signaling on the cytoskeleton, human embryonic kidney (HEK)-293 cells stably transfected with CaR (CaR-HEK) were incubated with CaR agonists in serum-free medium for up to 3 h. Addition of the calcimimet...

The murine BALB/c 3T3 fibroblast clone SV-T2 (3T3 cells) expresses receptors for the nonapeptide bradykinin. In these cells, bradykinin stimulates both inositol phosphate (InsP) formation and arachidonic acid release by independently activating phospholipase C and phospholipase A2, respectively. These actions of bradykinin are mediated by a receptor(s) coupled to pertussis toxin-insensitive gua...

s | 51 who represented 3.8% of the entire claims for outpatient medical services in 2010.The measurement units used for psychotropic drugs were drug items and the number of defined daily doses (DDDs). Besides overall description, the data of psychotropic drugs were analyzed by stratifying patient’s age and chemical subgroup of antipsychotic drugs. Results: Prescription of psychotropic drug item...

Orientia (O.) tsutsugamushi-induced scrub typhus is endemic across many regions of Asia and the Western Pacific, where an estimated 1 million cases occur each year; the majority of patients infected with O. tsutsugamushi end up with a cytokine storm from a severe inflammatory response. Previous reports have indicated that blocking tumor necrosis factor (TNF)-α reduced cell injury from a cytokin...

Cross-linking of CD45 induced capping and physical sequestration from CD22 leading to an increase in tyrosine phosphorylation of CD22 and SHP-1 recruitment. Additionally, CD22 isolated from a CD45-deficient B cell line exhibited increased basal/inducible tyrosine phosphorylation and enhanced recruitment of SHP-1 compared with CD22 isolated from CD45-positive parental cells. Subsequent experimen...

s | 51 who represented 3.8% of the entire claims for outpatient medical services in 2010.The measurement units used for psychotropic drugs were drug items and the number of defined daily doses (DDDs). Besides overall description, the data of psychotropic drugs were analyzed by stratifying patient’s age and chemical subgroup of antipsychotic drugs. Results: Prescription of psychotropic drug item...

BACKGROUND Granulosa cells play an important endocrine role in folliculogenesis. They mobilize Ca2+ from intracellular stores by a coordinated action between 1,4,5 inositol trisphosphate and ryanodine receptors (IP3R and RyR). The aim of this study was to explore the isoforms of IP3Rs expressed in mouse C57BL/6 NHsd granulosa cells, characterizing their intranuclear localization and the relatio...

s | 51 who represented 3.8% of the entire claims for outpatient medical services in 2010.The measurement units used for psychotropic drugs were drug items and the number of defined daily doses (DDDs). Besides overall description, the data of psychotropic drugs were analyzed by stratifying patient’s age and chemical subgroup of antipsychotic drugs. Results: Prescription of psychotropic drug item...

Circulating free fatty acids (FFAs) are elevated in various disorders like states of insulin resistance, and an increase of FFAs has been reported to be associated with endothelial dysfunction. To investigate the effect of FFAs on vascular endothelial cells, we measured nitric oxide (NO) release and intracellular free calcium concentration ([Ca2+]i ) in cultured bovine aortic endothelial cells ...