The biopharmaceutical market is one of the fastest growing biotechnology markets. In order to ensure affordable and reliable therapeutics, process has be closely monitored. Capillary electrophoresis (CE) proven a valuable technique for analysis product concentration, critical quality attributes, process-related impurities, nutrients metabolites in cell culture medium. zone electrophoresis, capi...

Please address correspondence to Frank A. Gomez at [email protected]. nteractions between biological species are essential to life and are involved directly in many of the enzyme-based reactions involved in cell division, cell death, and cell transformations. These biological interactions are important in the initiation, progression, and harmful effects of human disease including Parkinson...

Humic substances (HS) which are widespread in the soil, present structural complexity and polyelectrolyte properties. Many techniques and methods have been used to obtain more information about them, as reviewed by Davies and Ghabbour in 1999. The characterization of HS has been the focus of intense research for many years because the organic matter in soil contributes to the quality of the soi...

Many researchers have invested considerable efforts toward improving capillary electrophoresis (CE)-mass spectrometry (MS) systems so they can be applied better to standard analyses. This review highlights the developments in CE-MS of proteins and peptides over the last five years. It includes the developments in interfaces, sample-enrichment techniques, microfabricated devices, and some applic...

After having grown through the stages of technique development and instrumentation availability, capillary electrophoresis has reached the stage of applications. This review attempts to show the potential of this technique for biomedical analysis. Rather than going into a detailed description of the technical details of the separation conditions suitable for the separation of a particular categ...

Partial-filling affinity capillary electrophoresis (PFACE) is used to examine the binding interactions between two model biological systems: D-Ala-D-Ala terminus peptides to the glycopeptide antibiotic vancomycin (Van) from Streptomyces orientalis, and arylsulfonamides to carbonic anhydrase B (CAB, EC 4.2.1.1, bovine erythrocytes). Using these two systems, modifications in the PFACE technique a...

We investigate electrokinetic transport of nanoparticles and biomolecules in nano-scale channels exhibiting novel features in comparison to microscale channels. Recent experimental work provides information about how the mobility and diffusivity depend sensitively and nonmonotonically on pH, ionic valence, and channel size. We discuss a TIRFM platform in which single quantum dot nanoparticles a...

We recently reported acupuncture sample injection that leads to reproducible injection of nL-scale sample segments into a polydimethylsiloxane (PDMS) microchannel for microchip capillary electrophoresis. The advantages of the acupuncture injection in microchip capillary electrophoresis include capability of minimizing sample loss and voltage control hardware and capability of introducing sample...

A capillary electrophoresis method was used for assay of some degradation products of carvedilol. The optimized parameters were as; running buffer 80 mM acetate dissolved in methanol/ethanol mixture (65:35% v/v), applied voltage of 19 kV, temperature is 20 ºC and the wavelength range of 200-350 nm. The results indicate that the proposed capillary electrophoresis method could effectively separat...