In the efforts to achieve zero-emission transportation, hydrogen offers a valid choice as complete replacement of gasoline. Adapting spark ignition (SI) engines this alternative fuel can be implemented with relatively minor changes and limited investment in added components. The conversion small-size passenger car fueling was evaluated initially from perspective achievable range peak power. Ove...

We provide characterization of the strong termination property of the CCV λμ-calculus introduced in the first part of the series of the paper. The calculus is complete with respect to the standard CPS semantics. The union-intersection type systems for the calculus is developed in the previous paper. We characterize the strong normalizability of terms of the calculus in terms of the CPS semantic...

The trans Golgi network (TGN) of plant cells sorts and packages Golgi products into secretory (SV) and clathrin-coated (CCV) vesicles. We have analyzed of TGN cisternae in Arabidopsis root meristem cells by cell fractionation and electron microscopy/tomography to establish reliable criteria for identifying TGN cisternae in plant cells, and to define their functional attributes. Transformation o...

Research showing that women commit high rates of intimate partner violence (IPV) against men has been controversial because IPV is typically framed as caused by the patriarchal construction of society and men's domination over women. Johnson's (1995) typology of common couple violence (CCV) and intimate terrorism (IT) attempted to resolve this controversy, but he maintained that IT was caused b...

The lipid mediator sphingosine 1-phosphate (S1P) regulates a wide range of cellular activities, including vascular maturation, angiogenesis, and immune-cell trafficking. Among the five known receptors for S1P (S1PR1-S1PR5), S1PR1 is a critical regulator of lymphocyte trafficking: its signaling is required for lymphocyte egress from lymphoid organs, while its down-modulation by agonist-induced i...

Although adaptor protein complex 1 (AP-1) and Golgi-localized, γ ear-containing, ADP-ribosylation factor-binding proteins (GGAs) are both adaptors for clathrin-mediated intracellular trafficking, the pathways they mediate and their relationship to each other remain open questions. To tease apart the functions of AP-1 and GGAs, we rapidly inactivated each adaptor using the "knocksideways" system...

Galpha-interacting protein (GAIP) is a member of the RGS (regulators of G protein signaling) family, which serve as GAPs (GTPase-activating proteins) for Galpha subunits. Previously, we demonstrated that GAIP is localized on clathrin-coated vesicles (CCVs). Here, we tested whether GAIP-enriched vesicles could accelerate the GTPase activity of Galphai proteins. A rat liver fraction containing ve...

Within the clathrin-coated vesicle (CCV) cycle, coat assembly drives the internalization of receptors from the cell surface and disassembly allows for the processing of internalized ligands. The heat shock cognate protein, hsc70, has been implicated in regulating coat disassembly. We find that in cells overexpressing ATPase-deficient hsc70 mutants, uncoating of CCVs is inhibited in vivo, and th...

The CV transition (or PIVOT) is an area at which the maximum of new spectral information coincides with rapidly rising intensity. These acoustic characteristics of PIVOTs are among the most salient events in speech [1,2]. It has been shown [3] that spectral changes whose response in the auditory nerve is predominantly an onset of firing (like in the CV transition) are much more perceptually sal...