Introduction: Stromal cells interact with tumor cells and play an important role in cancer invasion and metastasis. Tumor-associated macrophages (TAMs) can exhibit M1 phenotype, important immune effector cells, or M2 phenotype, able to suppress the function of M1 macrophages and influence angiogenesis and tissue repair. The CD68 antibody recognizes M1 and M2 macrophages, whereas CD163 antibody ...

Dopamine replacement therapy for Parkinson’s disease is achieved using L-DOPA or dopamine D2/3 agonists, such as ropinirole. Here, we compare the effects of and ropinirole, alone in combination, on patterns glial microvascular reactivity striatum. Rats with unilateral 6-hydroxydopamine lesions were treated therapeutic-like doses (6 mg/kg), an equipotent L-DOPA-ropinirole combination (L-DOPA 3 m...

Abstract The immunological status of human meningiomas is not well understood, hindering the development rational immunotherapeutic strategies. We measured levels PD-L1, PD-L2, and immune cell subsets using multiplex quantitative immunofluorescence in a tissue microarray composed 73 (56 WHO Grade 1, 13 2, 4 3). analyzed tumor-infiltrating populations, T-cell activation/dysfunction, macrophage p...

objective(s): occludin is essential for proper assembly of tight junctions (tjs) which regulate paracellular endothelial permeability. omega-3 polyunsaturated fatty acids (ω-3 pufa) protect endothelial barrier function against injury. materials and methods: we examined anti-inflammatory effect of ω-3 pufa intake (30 mg/kg/day for 10 days) on expression and location of occludin in the aorta of a...

HMG-CoA reductase inhibitors (statins) decrease atherosclerosis by lowering low-density-lipoprotein cholesterol. Statins are also thought to have additional anti-atherogenic properties, yet defining these non-conventional modes of statin action remains incomplete. We have previously developed a novel mouse transplant model of atherosclerosis regression in which aortic segments from diseased don...

Macrophage polarization is increasingly recognised as an important pathogenetic factor in inflammatory and neoplastic diseases. Proinflammatory M1 macrophages promote T helper (Th) 1 responses and show tumoricidal activity. M2 macrophages contribute to tissue repair and promote Th2 responses. CD68 and CD163 are used to identify macrophages in tissue sections. However, characterisation of polari...

CONTEXT Inflamed seborrheic keratoses are generally associated with the accumulation of variable numbers of lymphocytes and histiocytes in the superficial dermis. The precise immunologic mechanism of this histologic phenomenon is not known CASE REPORT A 62-year-old male presented with a patch on the right neck with additional features of inflammation. Skin biopsies for hematoxylin and eosin e...

We have previously shown that mouse atherosclerosis regression involves monocyte-derived (CD68+) cell emigration from plaques and is dependent on the chemokine receptor CCR7. Concurrent with regression, mRNA levels of the gene encoding LXRalpha are increased in plaque CD68+ cells, suggestive of a functional relationship between LXR and CCR7. To extend these results, atherosclerotic Apoe-/- mice...

To understand the significance of the reported depot differences in preadipocyte dynamics, we developed a procedure to identify committed preadipocytes in the stromovascular fraction of fresh human adipose tissue. We documented that adipocyte fatty acid binding protein (aP2) is expressed in human preadipocyte clones capable of replication, indicating that can be used as a marker of committed pr...

Background—We previously showed that the progression of atherosclerosis in the Reversa mouse (Ldlr / Apob100/100Mttpfl/fl Mx1Cre / ) was arrested when the hyperlipidemia was normalized by inactivating the gene for microsomal triglyceride transfer protein. Here, we tested whether atherosclerosis would regress if the lipid levels were reduced after advanced plaques formed. Methods and Results—Rev...