With the aim of carrying out chimaerism and somatic cell-midblastula transition (MBT) embryos co-culture experiments in freshwater fish species, we evaluated the effect of osmolarity and composition of two media commonly used in cell fish culture on MBT zebrafish embryos and their further development and survival. To this end, wild zebrafish dechorionated embryos in midblastula stage were cultu...

Background The pharmacological manipulation of the interaction between the immune system, fibroblasts and cancer cells has been proven to be a key target for cancer therapy. Based on our previously developed co-culture system, this work aims to develop an innovative 3D cell culture system consisting of immune cells, fibroblasts and cancer cells and to investigate then influence of the tumor mic...

We have earlier proposed that a cell-cell interaction, mediated by the specific binding of molecules of the beta-amyloid precursor protein (beta-APP) on one cell surface with molecules of presenilin (PS) on the other cell surface, is a required initial step in the ultimate production of beta-amyloid (Abeta) from beta-APP. Abeta is widely believed to be the neurotoxic agent in Alzheimer's diseas...

BACKGROUND Positive feedback is a common motif in gene regulatory networks. It can be used in synthetic networks as an amplifier to increase the level of gene expression, as well as a nonlinear module to create bistable gene networks that display hysteresis in response to a given stimulus. Using a synthetic positive feedback-based tetracycline sensor in E. coli, we show that the population dyna...

background: sickle-cell disease (scd) is an inherited hemoglobin childhood disorder, frequently complicated by pulmonary hypertension and cardiac involvement. cardiovascular events and complications are the leading cause of mortality and morbidity in patients with scd. tissue doppler imaging and the myocardial performance index (tei index), are simple indices for the assessment of the cardiac f...

objective: we studied both the presence of some carbohydrate compounds in a three-dimensional (3d) matrix harvested from human gingiva and the cell behavior in this matrix. materials and methods: in this experimental research, in order to prepare 3d scaffolds, human palatal gingival biopsies were harvested and physically decellularized by freeze-thawing and sodium dodecyl sulfate (sds). the sca...